【作者】 刘丹；

【导师】 南极星；

【作者基本信息】 延边大学 ， 药物分析学， 2010， 硕士

【摘要】 目的：考察从土壤中分离出的微生物(YS2-2)对人参总皂苷的生物转化作用；并考察转化产物作对Hela(宫颈癌细胞)的抑制以及对S180小鼠肉瘤的抑制作用。论文还考察了发酵产物对APAP(对乙酰氨基酚)所致小鼠肝损伤的保护作用。方法：从土壤中分离筛选出人参皂苷生物转化高效菌株YS2-2,对人参总皂苷进行生物转化。转化条件为：R2A培养基,30℃震荡培养4天。通过TLC(薄层色谱法)、HPLC(高效液相色谱法)检测其转化产物；将转化产物用DMSO(二甲基亚砜)配制成浓度分别为200μg/mL、100μg/mL、50μg/mL、25μg/mL。采用MTT法测定对宫颈癌细胞的抑制率；用腹水型S180肿瘤细胞,调整细胞浓度为1×107个／mL。将稀释的瘤液接种于小鼠右腋下,每只小鼠0.2 mL造模24小时后,灌胃给予发酵人参300 mg/kg, 150 mg/kg,50 mg/kg,7天后称定肿瘤、胰腺及脾脏重量,考察发酵人参对S180的抑制作用及对免疫器官的影响。在考察发酵人参对肝保护的实验中,将药物配置成300mg/kg,200 mg/kg,1000 mg/kg,给予APAP所致小鼠肝损伤模型,测定其血清中的ALT(谷丙转氨酶)、AST(谷草转氨酶)值以及肝脏匀浆中的SOD(超氧化物歧化酶)、GSH(谷胱甘肽)值。结果：微生物YS2-2对人参皂苷具有较强的转化能力,其转化产物为人参稀有皂苷Rg3和C-K。不同浓度的发酵人参对宫颈癌细胞的抑制率分别为29.48%,31.65%,34.53%,68.22%。大中小剂量对S180小鼠肉瘤的抑制率分别为53.03%,34.51%,27.67%。对APAP所致小鼠肝损伤模型中,在给予300,200,100 mg/kg发酵人参后,可以降低因APAP导致的肝损伤小鼠血清中AST, ALT活力及肝脏中SOD、GSH水平。结论：从土壤中分离出的微生物YS2-2能够转化人参皂苷；并且转化产物对宫颈癌细胞有抑制作用,低浓度的抑制作用最为明显；对S180小鼠肉瘤细胞的抑制作用,在高浓度是最为明显；发酵人参对APAP所致小鼠肝损伤有一定的保护作用。更多还原

【Abstract】 AIM:To investigate the transformation of YS2-2 microorganism to ginsenosides; Then, assay the execution of fermentation of ginsenosides when they kill anticancer cells by MTT method. Investigate the effect on the sarcoma180 bearing in mice treated by fermentation of ginsenosides. Finally, review the protective of the fermentation products on APAP induced acute liver injury on mice. METHODS:In this paper, we transformed the ginsenoside with the YS2-2 for 4 days. The fermentation products tested with TLC and HPLC method. This study was to investigate the fermentation of ginsenosides with different concentration(200μg/mL、100μg/mL、50μg/mL、25μg/mL) to cervical cancer cells using in vitro MTT assay chemosensitivity test. The antitumor effect of fermentation of ginsenosides on the S180 bearing in mice was evaluated by tumor weight, spleen weight and thymus weight. And then we gave the fermented ginsenoside to the mice, which injured by APAP, by O.P. Test the content of AST and ALT in serum as well as the effects of SOD and GSH level changes in the mouse liver. RESULTS: After transforming, the content of Rg3 raised obviously and a small quantity of compound K. The inhibition ratio of fermentation of ginsenosides on Hela with different concentration were 29.48%. 31.65%,34.53%,68.22%respectively. And the inhibition ratio of the drug on S180 were 53.03%,34.51%,27.67%respectively. Finally, the fermentation products could reduce the content of ALT, AST in serum of acute liver injury, and the SOD, GSH levels are also improved in liver. In particular, the fermentation of middle concentration has the most significant effect. CONCLUSION: We found that the YS2-2 can transform the ginsenosides in vitro. And fermentation of ginsenosides could inhibit the Hela growth, also the s180 tumor. Meanwhile, we also did the experience that the transformation product whether can protect the liver injured by APAP. We suggest that it has a protective effect on the APAP induced acute liver injury on mice. The data is the evidence that the transformation of ginsenoside can induce the apoptosis.更多还原