【作者】 王鹏；

【导师】 赖真；

【作者基本信息】 暨南大学 ， 中西医结合临床， 2010， 硕士

【摘要】 目的：拟观察黄芪注射液和红花注射液对SD大鼠局灶性脑缺血再灌注模型神经细胞凋亡、转化生长因子-β1(TGF-β1, transforming growth factor-β1)和B细胞淋巴瘤／白血病-2(B-cell lymphoma/leukemia-2, bcl-2)表达的影响,来探讨黄芪注射液联合红花注射液治疗脑缺血性中风的作用机制。方法：将SD雄性大鼠随机分为假手术组、模型组、黄芪组、红花组、黄芪加红花组,各药物干预组分别给予腹腔注射相应药物,假手术组与模型组分别予腹腔注射等量生理盐水；采用线栓法制备局灶性脑缺血再灌注模型。各组干预时间均为手术前12h,30min,术后每隔12h给药一次。分别在缺血再灌注12h,24h,48h对其神经系统体征进行客观评分后断头处死。行HE染色观察各时间点脑组织病理学形态的变化；并运用TUNEL法观察缺血再灌注24h神经细胞凋亡的情况；免疫组化的方法检测TGF-p1、bcl-2的表达,在光镜下分别计数其阳性细胞数。结果：与模型组比较,黄芪加红花组、黄芪组和红花组均能改善脑缺血再灌注模型SD大鼠神经损伤症状；减轻脑缺血再灌注时的病理损伤；减少凋亡阳性细胞的表达；增加TGF-p1、bcl-2阳性细胞在各时间点的表达(p<0.05),且黄芪加红花组与黄芪组、红花组比较,各时间点TGF-β1、bcl-2阳性细胞数增多更明显,差异有显著性(p<0.05)。结论：黄芪注射液联合红花注射液能减轻脑缺血再灌注大鼠脑神经细胞凋亡,其机制可能和其上调TGF-β1、bcl-2的表达有关；黄芪注射液与红花注射液联用具有协同效应,治疗效果优于各自单独使用。更多还原

【Abstract】 Objective:To study the therapeutic effects of radix astragali injection(RA) and safflor injection(SI) on Transforming growth factor-β1 (TGF-β1), B-cell lymphoma/leukemia-2 (Bcl-2) and apoptosis of nerve cells in rats brains following local cerebral ischemia/reperfusion.Methods:Male adult SD rats were randomly divided into five groups:the sham-operated group, the model group, the RA group,the SI group and the RA+SI group. The model of middle cerebral artery occlusion(MCAO) was established by thread ligation method, and these rats were injected intraperitoneally corresponding medicine in different groups at 12h,30min before operation and every other 12h after operation. In sham-operated group and the model group, equally dose saline was injected intraperitoneally. Neurological system symptoms were evaluated at 12h,24h,48h after reperfusion. Then the rats were decapitated at 12h,24h, and 48h after reperfusion, and rats brains were taken for histopathological, TUNEL and immunohistochemistry examinations. Positive reacted cells of apoptosis, TGF-β1 and Bcl-2 are counted under light microscope at different time points of ischemia/reperfusion.Results:The score of neurological system damage symptoms and the examination of histopathological, TUNEL, immunohistochemistry show that the cerebral ischemic damage in the RA group, the SI group and the RA+SI group was significantly milder than that in the model group(p<0.05). Positive immune reacted cell amounts of TGF-β1 and Bcl-2 are increased in RA+SI group as compared with the RA group and the SI group in 12h,24h,48h ischemia/ reperfusion(p<0.05).Conclusions:The cooperation protective effect of RA and SI injection therapy against cerebral ischemia reperfusion injury might be associated with increasing the positive expressions of TGF-β1 and Bcl-2. Moreover, The cooperation therapeutic effect of RA and SI is superior the unity therapeutic effect of RA or SI.更多还原