【作者】 姚磊；

【导师】 潘新良；

【作者基本信息】 山东大学 ， 耳鼻咽喉头颈外科， 2009， 硕士

【摘要】 目的:研究下咽鳞状细胞癌组织中神经迁移蛋白Slit2的表达及其与微血管密度的关系,探讨两者对下咽鳞状细胞癌的生物学行为的影响。方法:免疫组织化学Envision二步法检测66例下咽癌组织和42例癌旁组织中Slit2蛋白的表达水平及CD34标记的微血管密度;并应用RT-PCR法检测新鲜组织中Slit2 mRNA的表达。观察不同的临床病理学行为下Slit2表达的变化及MVD的差异,研究下咽癌组织中Slit2的表达与MVD的关系及对肿瘤生物学行为的影响。结果:Slit2蛋白在下咽鳞状细胞癌和癌旁组织中的表达率分别为65.2%和40.5%,两者的表达差异有统计学意义(x~2=6.329,P＜0.05),阳性表达部位主要位于肿瘤细胞质内,棕黄色,颗粒状,在鳞癌癌巢的中心部位较周边部位表达更强;Slit2 mRNA在下咽鳞状细胞癌和癌旁组织中的差异性表达亦具有统计学意义(t=8.230,P＜0.05),与免疫组织化学分析结果相符;下咽鳞状细胞癌和癌旁组织中MVD计数分别为26.68±5.97,19.92±3.73,两者存在显著性差异(t=7.23l,P＜0.01)。Slit2的表达与肿瘤大小,TNM分期,淋巴节转移显著相关;MVD计数与肿瘤大小,浸润深度,TNM分期和淋巴节转移显著相关;两者与肿瘤的发病年龄,部位,临床病理分化程度均无相关。66例下咽癌组织中,Slit2蛋白表达阳性43例,其MVD计数为27.87±5.61;Slit2蛋白表达阴性23例,其MVD计数为24.47±6.12,两者MVD计数差异具有统计学意义(t=2.273,P＜0.05)。肿瘤组织中Slit2蛋白的表达与MVD计数呈正相关关系(r_s=0.430,P＜0.05)。结论:1.下咽鳞状细胞癌组织中Slit2蛋白及mRNA的表达和MVD计数均较癌旁组织的相应指标计数有所增高,并且在肿瘤组织中两者表达呈正相关关系;2.下咽鳞状细胞癌组织中Slit2蛋白及mRNA的表达和MVD计数与肿瘤的发生、发展、侵袭和淋巴结转移等临床特征密切相关,其机制可能为Slit2基因介导的肿瘤新生血管的形成,从而影响了肿瘤的生物学行为;3.Slit2蛋白及mRNA的表达强度及MVD的计数值可以作为判断下咽鳞状细胞癌的生长、浸润及转移能力的重要指标。更多还原

【Abstract】 Objective: To investigate the expression of Slit2 and its correlation with the microvessel density in human hypopharyngeal squamous cell carcinomas, and to analyze their influences to the biological behaviors of human hypopharyngeal squamous cell carcinomas.Method: The expression of Slit2 protein and CD34-labeled microvessel density (MVD) were measured by immunohistochemical staining (Envision). The expressions of Slit2 mRNA in 24 cases of fresh tissues were measured by reverse-transcript polymerase chain reaction. The relations between the above expressions and the clinical features of these patients were analyzed by statistical methods.Results The positive rates of Slit2 in cancerous tissues and Para-cancer tissues were respectively 65.2% and 40.5% and the difference between them had statistical significance (X~2=6.329, P<0.05).The Slit2 mRNA expressions between them also had statistical significance (t=8.230, P<0.05). The expression rate of Slit2 and the enumerations of MVD were significantly associated with tumor size, TNM stage and lymph node metastasis. The enumerations of MVD were high in Slit2-positive tissues, and the difference had statistical significance (t=2.273, P<0.05) . The expression of Slit2 had positive correlation with the MVD (r_s=0.430, P<0.05) .Conclusion: 1. The expression of Slit2 and the enumerations of MVD were significantly higher in hypopharyngeal squamous cell carcinomas than that in normal mucosa. The relation between them in cancerous tissues was positive.2. The expression of Slit2 and the enumerations of MVD had significant relations with the tumors’ clinical features, such as invasion and lymph node metastasis. The mechanism might be Slit2-mediated new vessels formation, which affected the tumors’ biological behaviors.3. The expression intensity of Slit2 and the enumerations of MVD can be used as an important index for the capacity of growth, invasion and metastasis in the hypopharyngeal squamous cell carcinomas.更多还原