【作者】 兰花；

【导师】 张少容；

【作者基本信息】 南昌大学 ， 耳鼻喉—头颈外科学， 2009， 硕士

【摘要】 目的:探讨OPN和CD44v6在喉癌组织中的表达、相互关系及临床意义。方法:应用免疫组织化学EliVision二步法检测44例喉鳞状细胞癌组织、10例癌旁正常组织标本中OPN和CD44v6蛋白的表达,并分析二者与喉癌临床病理特征的关系及二者表达的相关性。结果:①喉癌组织中OPN和CD44v6的阳性表达率分别为75.0% (33/44)和68.2%(30/44),显著高于癌旁正常组织的30.0%(3/10)和20.0%(2/10)(均P <0.05);②喉癌组织中,OPN的表达与喉癌临床分期,病理分级及淋巴结转移有关,CD44v6的表达与喉癌临床分期,病理分级及淋巴结转移有关(均P <0.05),而二者的表达与患者年龄、性别、肿瘤位置无关(均P >0.05);③喉癌组织中OPN和CD44v6的表达呈正相关(rs=0.584,P <0.01)。结论:喉癌组织中OPN和CD44v6的高表达与喉癌的发生、发展及转移有关,OPN可能通过上调CD44v6的表达来促进喉癌的发展,联合检测可作为判断喉癌发生及转移潜能的参考指标之一。更多还原

【Abstract】 Objective:To investigate the expressions,relationships and clinical significance of OPN and CD44v6 in laryngeal squamous cell carcinomas (LSCC).Method:Immunohistochemical technology(EliVision two steps) was applied to detect the expressions of OPN and CD44v6proteins in 44 cases of LSCC tissue and 10 cases of the tumor adjacent tissue,and to study the relationships of OPN and CD44V6 proteins expressions with clinical features in patients with LSCC,the correlation of two proteins expressions was also analyzed.Rusult:①The positive rates of OPN (75.0%,33/44) and CD44v6 (68.2%,30/44) in LSCC tissue were respectively higher than that in the tumor adjacent tissue( 30.0%,3/10),(20.0%,2/10),P <0.05,respectively;②The positive rate of OPN was significantly correlated with the clinical staging,the pathological grading,the metastasis to cervical lymph node( P <0.05,respectively),the positive rate of CD44v6 was correlated with the clinical staging,the pathological grading,the metastasis to cervical lymph node ( P <0.05),but the positive rates of OPN and CD44v6 were not correlated with patient’s age,sex,tumor location ( P >0.05,respectively).③The expression of OPN was positively correlated with CD44V6 in LSCC (rs = 0.584,P <0.01).Conclusion:The over-expression of OPN and CD44v6 were correlated with occurence and development of LSCC,OPN may promote the development in LSCC by up-regulating the expression of CD44V6,It is suggested that OPN and CD44v6 can be a useful marker for predicting tumorigenesis and metastasis potential of LSCC.更多还原