【作者】 钟启宝；

【导师】 冯晓辉；

【作者基本信息】 南华大学 ， 外科学， 2008， 硕士

【摘要】 目的探讨EphA2、E-cadherin在喉鳞状细胞癌中的表达和其与喉鳞状细胞癌临床病理特征的关系,以及EphA2、E-cadherin在喉鳞状细胞癌中表达的相关性。方法收集我院2002～2006年病理科保存、外科手术切除的喉鳞状细胞癌石蜡包埋标本62例,并在以上标本中确定为癌旁组织正常鳞状上皮60例。分别记录患者的年龄、性别、组织分化程度、有无颈淋巴结转移及TMN分期。全部标本均经常规病理确诊。病理标本做连续切片,厚4um,用链霉素抗生物素蛋白-过氧化物酶链接法(SP法)做EphA2、E-cadherin标记。免疫组化结果判定综合染色强度及阳性细胞数两方面进行分析。对EphA2、E-cadherin在喉鳞状细胞癌、癌旁组织正常鳞状上皮中的表达分别进行统计学分析;对EphA2、E-cadherin的表达分别与喉鳞状细胞癌各临床病理特征进行统计学分析;以及EphA2和E-cadherin在喉鳞状细胞癌中表达的相关性进行统计学分析。结果一、EphA2在喉鳞状细胞癌中表达的阳性率为82.26%(51/62),在癌旁组织正常鳞状上皮中表达阳性率为36.67%(22/60),EphA2在喉鳞状细胞癌中的表达明显高于癌旁组织正常鳞状上皮(p<0.01)。二、E-cadherin在喉鳞状细胞癌中表达阳性率为45.16%(28/62),在癌旁组织正常鳞状上皮表达的阳性率为93.33%(56/60), E-cadherin在喉鳞状细胞癌中的表达明显低于癌旁组织正常鳞状上皮(p<0.01)。三、EphA2的表达与喉鳞状细胞癌的组织分化程度、颈淋巴结转移以及临床分期,差别具有显著性(P<0.05);但与喉鳞状细胞癌患者的年龄、性别差别无统计学意义(P>0.05)。四、E-cadherin的表达与喉鳞状细胞癌的组织分化程度、颈淋巴结转移以及临床分期,差别具有显著性(P<0.01);但与喉鳞状细胞癌患者的年龄、性别差别无统计学意义(P>0.05)。五、喉鳞状细胞癌中EphA2和E-cadherin的表达呈负相关(r=-0.325,P<0.01)。结论一、EphA2在喉鳞状细胞癌中呈高表达,其高表达与喉鳞状细胞癌的组织分化程度、颈淋巴结转移以及临床分期有关,而与喉鳞状细胞癌患者的年龄、性别无关。EphA2可能在喉鳞状细胞癌的发生发展中起一定的促进作用,EphA2的高表达可能提示了喉鳞状细胞癌的恶性程度。二、E-cadherin在喉鳞状细胞癌中呈低表达,其低表达与喉鳞状细胞癌的组织分化程度、颈淋巴结转移以及临床分期有关,而与喉鳞状细胞癌患者的年龄、性别无关。E-cadherin可能在喉鳞状细胞癌发生发展中起一定的抑制作用,E-cadherin的低表达可能提示了喉鳞状细胞癌的恶性程度。三、喉鳞状细胞癌中EphA2和E-cadherin表达呈负相关。更多还原

【Abstract】 Purpose To study the expression of EphA2 and E-cadherin in laryngeal squamous cell carcinoma(LSCC) ,and the relationship of the expression with the clinical pathology characteristics of LSCC ,and also to understand the correlation between the expression of EphA2 and E-cadherin in LSCC.Methods 62 samples of paraffin wax embed laryngeal squamous cell carcinoma were collected for the present study.These samples were cut off from surgical operations and stored in pathologic section of the people’s hospital of Hunan province from 2002 to 2006. 60 samples of normal squamous epidermis near cancer were selected.The patients’ age, sex, differentiation, cervical lymph node matastasis and TNM stage were recorded.All samples were recuted to diagnose. The pathological specimen was cut in the thickness of 4um. Streptavidin peroxidase (SP) immunohistochemistry staining for EphA2 and E-cadherin were made.The result of immunohistochemistry were determined by the intensity of staining and the number of positive cells.The expressions of EphA2 and E-cadherin in LSCC,and normal squamous epidermis near cancer were analyzed.The relationship with the expression of EphA2 and E-cadherin with the clinical pathology characteristics were done. and the relevance of the expression of EphA2 and E-cadherin in LSCC was analysed.Result1 The positive rate of EphA2 expression was 82.26%(51/62) in LSCC,and in normal squamous epidermis near cancer was 36.67%(22/60). The expression of EphA2 in LSCC was significantly higher than that in Lnormal squamous epidermis near cancer (P<0.01). 2 The positive rate of E-cadherin expression was 45.16%(28/62)in LSCC,and in normal squamous epidermis near cancer was 93.33%(56/60). The expression of E-cadherin in LSCC was significantly lower than that in Lnormal squamous epidermis near cancer (P<0.01).3 There was a significant correlation between the expression of EphA2 in LSCC with the type of pathology,cervical lymph node matastasis and the tumor TNM stage (P<0.05). However There was no significant correlation between the expression of EphA2 with the patients’ age and sex (P>0.05) .4 There was a significant correlation between the expression of E-cadherin in LSCC with the type of pathology, cervical lymph node matastasis and the tumor TNM stage(P<0.01). while There was no significant correlation between the expression of E-cadherin with the patients’ age and sex(P>0.05) .5 In LSCC, The expression of EphA2 was inversely correlated with that of E-cadherin(r=-0.325,P<0.01).Conclusion1 The expression of EphA2 was significantly increased in LSCC .Higher EphA2 expression was significantly associated with the type of pathology ,cervical lymph node matastasis and the tumor TNM stage,but not related with the patients’ age and sex.EphA2 may accelerate the genesis or progress of LSCC. Higher EphA2 expression may imply more aggressive malignant behaviors of LSCC.2 The expression of E-cadherin was significantly reduced in LSCC.Dscending E-cadherin expression was significantly associated with the type of pathology ,cervical lymph node matastasis and the tumor TNM stage,but not related with the patients’ age and sex. E-cadherin may inhibit the genesis or progress of LSCC. Dscending E-cadherin expression may imply more aggressive malignant behaviors of LSCC.3 In LSCC, The expression of EphA2 and E-cadherin were nagatively correlated.更多还原