【作者】 刘媛；

【导师】 邵乐南；

【作者基本信息】 华中科技大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:肿瘤是涎腺组织中最常见的疾病,而肿瘤的发生是由于细胞周期中生长因子及其受体、细胞因子及众多癌基因、抑癌基因产物等对DNA代谢的调节发生改变。表皮生长因子受体家族及其相关配体是调节正常细胞生长的重要信号系统,此系统的紊乱可导致细胞的恶性转化。本文旨在研究ErbB家族中表皮生长因子受体(EGFR)和C-erbB-2在涎腺肿瘤中的表达,探讨EGFR和C-erbB-2两者之间的关系及临床意义。方法:选取华中科技大学同济医学院附属同济医院涎腺肿瘤标本70例,其中良性肿瘤30例、恶性肿瘤40例。应用免疫组化S-P法检测石蜡包埋标本中的EGFR和C-erbB-2的阳性率。另取2例已知EGFR、C-erbB-2蛋白表达阳性的乳腺浸润性导管癌组织作阳性对照。结果:EGFR阳性表达定位于细胞膜或细胞质中;C-erbB-2阳性表达定位于细胞膜上。在口腔涎腺恶性肿瘤组织中,EGFR蛋白的阳性表达率为67.5%(27/40),高于口腔涎腺良性肿瘤组织43.3%(13/30)(P<0.05) ;C-erbB-2蛋白的阳性表达率为33.3%(10/30),显著高于口腔涎腺良性肿瘤组织6.7%(2/30)(P<0.05)。C-erbB-2与EGFR蛋白表达两者呈显著的正相关(r= 0.665,P<0.01)。结论:EGFR和/或C-erbB-2在涎腺肿瘤中均有表达,而且C-erbB-2与EGFR同时过度表达,可为涎腺肿瘤早期诊断、治疗及判断预后提供依据。更多还原

【Abstract】 ObjectiveTumor is the most common disease in salivary gland tissue, and the genesis is that growth factor and it’s receptor, cytokine, many oncogene protein and anti-oncogene protein which accommodate the metabolism of DNA has been changed. The epidermal growth factor receptor and it’s ligands are the important signal systems that regulate normal cell growth. Abnormal expression of the epidermal growth factor receptor and it’s ligands has been associated with malignant transformation. This text is to study the expression of human epidermal growth factor receptor (EGFR) and epidermal growth factor receptor 2 (C-erbB-2) in salivary gland tumor and discuss their clinical implications.MethodsAll specimens were obtained from patients of Tongji hospital of Tongji Medical College, Huazhong University of Science and Technology. Seventy cases of salivary gland tumor were included in this study. Among these cases, there were 30 samples of benign tumors, 40 samples of malignant tumors. SP immunohistochemical method was used to investigate the positive expression rates of EGFR and C-erbB-2 in paraffin-embedded salivary gland tumors. Meanwhile,we used lacteal gland infitrating ductal carcinoma sections which have positive expression of EGFR and C-erbB-2 as control group.ResultsEGFR positive staining was observed in cytomembrane or cytoplasm; C-erbB-2 positive staining was observed in cytomembrane. The positive expression rates of EGFR were 67.5% in malignant salivary gland tumor, whereas only 43.3% of benign were EGFR positive (P<0.05). The positive expression rates of C-erbB-2 were 33.3% in malignant salivary gland tumor, which was significantly higher than that benign tumor of 6.7%(P<0.05). The expression levels of C-erbB-2 and EGFR protein were positively correlated in malignant salivary gland tumor (r= 0.665,P<0.01).ConclusionThere existed positive expressions of EGFR and C-erbB-2 in salivary gland tumor, and co-overexpression of C-erbB-2 and EGFR might provide evidence for the early diagnosis and treatment of salivary gland tumor, as well as the assessment of prognosis.更多还原