【作者】 王怡丹；

【导师】 全炳武；

【作者基本信息】 延边大学 ， 作物栽培与耕作， 2008， 硕士

【摘要】 本试验以禾本科植物蒙古冰草(Agropyron mongolioum keng)、扁穗冰草(Agropyron cristatum(L.) Gaertn)和滨麦(Leymus mollis)为试材,用聚乙二醇6000(PEG6000)模拟土壤水分胁迫,对蒙古冰草、扁穗冰草和滨麦的种子发芽及生理生化反应等抗旱性指标进行对比研究,在此基础上对这三种植物在水分胁迫下的各种抗旱性指标之间进行相关分析,并对其抗旱性利用隶属函数法进行综合评价,研究内容及结论主要包含以下几个方面:1、在水分胁迫下蒙古冰草、扁穗冰草和滨麦种子的发芽率、发芽势、种子萌发胁迫指数明显下降。综合以上分析,蒙古冰草种子在萌发阶段的抗旱性优于扁穗冰草和滨麦。2、PEG6000模拟水分胁迫对蒙古冰草、扁穗冰草和滨麦的根部进行渗透胁迫,使供试材料产生一系列生理反应:叶片相对含水量、叶绿素显著下降,游离脯氨酸、可溶性糖显著增加,保护酶活性在轻度胁迫下升高,而中度或重度胁迫下活性降低或不变,细胞质膜透性明显增大,丙二醛随着水分胁迫的时间和胁迫程度的加深而增加。3、在PEG6000渗透胁迫24h后,蒙古冰草、扁穗冰草和滨麦相对含水量与质膜透性之间存在着极显著负相关关系(R=-0.76,P=0.01);相对含水量与丙二醛之间存在极显著负相关关系(R=-0.73,P=0.01):相对含水量与SOD之间存在显著负相关关系(R=-0.57,P=0.05);质膜透性与丙二醛之间存在极显著正相关关系(R=0.81,P=0.01);质膜透性与SOD之间存在极显著正相关关系(R=0.75,P=0.01);游离脯氨酸与叶绿素之间存在着显著负相关关系(R=-0.55,P=0.05);叶绿素与SOD之间存在着显著正相关关系(R=0.66,P=0.05);丙二醛与SOD之间存在显著正相关关系(R=0.63,P=0.05);丙二醛与POD之间存在显著正相关关系4、在PEG6000渗透胁迫48h后,蒙古冰草、扁穗冰草和滨麦相对含水量与质膜透性之间存在着显著负相关关系(R=-0.62,P=0.05):相对含水量与游离脯氨酸之间存在着显著负正关关系(R=-0.55,P=0.05);相对含水量与可溶性糖之间存在着显著负相关关系(R=-0.62,P=0.05):相对含水量与丙二醛之间存在极显著负相关关系(R=-0.73,P=0.01):质膜透性与丙二醛之间存在显著正相关关系(R=0.61,P=0.05):质膜透性与SOD之间存在极显著正相关关系(R=0.87,P=0.01);游离脯氨酸与叶绿素之间存在着显著负相关关系(R=-0.68,P=0.05);可溶性糖和丙二醛之间存在着显著正相关关系(R=0.63,P=0.05):叶绿素与SOD之间存在着显著正相关关系(R=0.60,P=0.05)。5、在PEG6000渗透胁迫72h后,蒙古冰草、扁穗冰草和滨麦相对含水量与质膜透性之间存在着显著负相关关系(R=-0.66,P=0.05);相对含水量与丙二醛之间存在极显著负相关关系(R=-0.76,P=0.01):质膜透性与丙二醛之间存在显著正相关关系(R=0.66,P=0.05);质膜透性与SOD之间存在显著正相关关系(R=0.61,P=0.05);游离脯氨酸与叶绿素之间存在着极显著负相关关系(R=-0.76,P=0.01):游离脯氨酸与丙二醛之间存在着显著正相关关系(R=0.62,P=0.05);游离脯氨酸与POD之间存在着极显著正相关关系(R=0.80,P=0.01);叶绿素与丙二醛之间存在着极显著负相关关系(R=-0.80,P=0.01);叶绿素与POD之间存在着极显著负相关关系(R=-0.79,P=0.01);丙二醛与POD之间存在显著正相关关系(R=0.56,P=0.05)。6、在24h、48h、72h时间段,对蒙古冰草、扁穗冰草和滨麦的水分胁迫所获得的各项抗旱指标来计算隶属函数值,作为抗旱性的综合评判。其抗旱排序为:滨麦＞蒙古冰草＞扁穗冰草。更多还原

【Abstract】 Studies on germination and physiological indices in seedings of Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis under PEG6000 water stress.The correlativity of some drought resistance indexes of 3 plants under water stress was analyzed.The drought resistance of 3 plants was evaluated by using subordination function method.The results are followings:1、The germination rate,germination vigor and seed germination drought resistance index decreased obviously under the water stress.Agropyron mongolioum keng was more strong drought resistance than the others in the germination stage.2、Under water stress conditions simulated by different concentration of polyethylene glycol 6000(PEG6000),physiological indices of drought resistance in Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis seeding were studied.The results showed that:the relative water contents in leaves、chlorophyl contents decreased,the proline content and soluble sugar increased;SOD and POD activity increased in the light stress,they were decreased or hold on in the medium or severe stress,the membrane permeability increased obviously,MDA increased too.3、The result of correlation analysis showed that there are positive or negative correlations between some physiology indexes of Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis under 24h water stress.The positive correlations are followings:between cell membrane permeability and MDA content (R=0.81,P=0.01);cell membrane permeability and SOD activity(R=0.75,P=0.01); chlorophyll content and SOD activity(R=0.66,P=0.05);MDA content and SOD activity (R=0.63,P=0.05);MDA content and POD activity(R=0.61,P=0.05).The negative correlations are followings:between relative water contents and cell membrane permeability(R=-0.76,P=0.01);relative water of leaves content and MDA content(R=-0.73,P=0.01);relative water of leaves content and SOD activity(R= -0.57,P=0.05);proline content and chlorophyll content(R=-0.55,P=0.05);MDA content and SOD activity(R=0.63,P=0.05);MDA content and SOD activity(R= 0.61,P=0.05).4、The result of correlation analysis showed that there are positive or negative correlations between some physiology indexes of Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis under 48h water stress.The positive correlations are followings:between cell membrane permeability and MDA content(R=0.61,P=0.05);cell membrane permeability and SOD activity(R=0.87, P=0.01);soluble sugar and MDA content(R= 0.63,P=0.05);chlorophyll content and SOD activity(R=0.60,P=0.05).The negative correlations are followings:between relative water contents and cell membrane permeability(R=-0.62,P=0.05);relative water contents and proline content(R=-0.55,P=0.05);relative water contents and soluble sugar(R=-0.62,P=0.05);relative water contents and MDA content(R=-0.73, P=0.01);proline content and chlorophyll content(R=-0.68,P=0.05).5、The result of correlation analysis showed that there are positive or negative correlations between some physiology indexes of Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis under 72h water stress.The positive correlations are followings:cell membrane permeability and MDA content(R =-0.66,P=0.05);cell membrane permeability and SOD activity(R=0.61,P=0.05); proline content and MDA content(R= 0.62,P=0.05);proline content and POD activity(R= 0.80,P=0.01).The negative correlations are followings:between relative water contents and cell membrane permeability(R=-0.66,P=0.05);relative water contents and MDA content(R=-0.76,P=0.01);proline content and chlorophyll content (R=-0.76,P=0.01);chlorophyll content and MDA content(R=0.80,P=0.01); chlorophyll content and POD activity(R=-0.79,P=0.01);MDA content and POD activity(R=0.56,P=0.05).6、The results of subordination function method of drought resistance for Agropyron mongolioum keng、Agropyron cristatum(L.) Gaertn and Leymus mollis in water stress 24h、48h、72h showed their resistance ability as following:Leymus mollis＞Agropyron mongolioum keng＞Agropyron cristatum(L.) Gaertn更多还原