【作者】 张锦玉；

【导师】 关立克；

【作者基本信息】 延边大学 ， 内科学， 2008， 硕士

【摘要】 目的:研究芝麻素对动脉粥样硬化兔模型血脂和细胞间黏附分子-1(ICAM-1)的影响作用,探讨芝麻素预防动脉粥样硬化形成的作用机制。方法:1.实验动物及分组:日本大耳白兔18只,用普通饲料适应性喂养一周后,按体重编号随机分成正常对照组(n=6)、动脉粥样硬化模型组(模型组)(n=6)和芝麻素组(n=6)。正常对照组喂饲普通饲料,模型组喂饲高胆固醇饲料,芝麻素组喂饲高胆固醇饲料的同时用芝麻素混悬液4mg/d灌胃。2.建立动物模型:实验开始(0周),模型组和芝麻素组经耳缘静脉一次性注射牛血清白蛋白250mg/kg体重,喂饲高胆固醇饲料,正常对照组经耳缘静脉一次性注射生理盐水5ml。3.实验方法:采用HE染色观察各组主动脉形态改变:采用氧化酶法于0W、5W末和8W末测定各组血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL);采用酶联免疫吸附法(ELISA)测定各组8W末血清ICAM-1水平。结果:1.主动脉形态学观察:光镜下芝麻素组主动脉粥样硬化改变明显轻于模型组2.血脂测定:芝麻素组血清TC、TG、LDL明显低于模型组(P＜0.01);3.ICAM-1测定:芝麻素组血清ICAM-1水平明显低于模型组(P＜0.01),与TC、LDL呈正相关。结论:1.芝麻素能有效地降低血清中TC、TG、LD L的浓度,减少内皮下脂质颗粒的蓄积,可能与芝麻素较强的抗氧化作用、抑制LDL氧化修饰生成ox-LDL有关。2.芝麻素能有效地降低血清ICAM-1的表达,表明芝麻素能够抑制细胞间黏附,减少炎症反应。3.芝麻素能够有效预防动脉粥样硬化形成,有望成为动脉粥样硬化一级预防的绿色保健药品。更多还原

【Abstract】 Objective:To investigate the expression of sesamin on cholesterol and serum intercellular adhesion molecule-lin atherosclerosis rabbits,and explore the effection of sesamin prevent atherosclerosis.Methods:1.Group:18 Japanese white rabbits were used.Rabbits fed normal diet were used as control group(n=6),and that fed high-cholesterol diet as atherosclerotic model group(n=6).The remained rabbits(n=6) were fed the high cholesterol diet containing sesamin(4mg/d,by stomach irrigating). 2.Model:Bovine serum albumin(250mg/kg) were used once by ear- edge intravenous injection to these rabbits fed high cholesterol diet at beginning of this study.3.Methods:The aortic fragments were cut and stained with hematoxylin to odserved the pathologic chang.Oxidase method was used to determine concentrations of total cholesterol(TC),triglycerides(TG),high-density lipoprotein(HDL) and low-density lipoprotein(LDL) at the time of 0,5th,8th week-end.ELISA method was used to determine serum inter-cellular adhesion molecule-1 of each group at the time of 8th week-end.Results:(1)The pathologic chang of arteria aorta endomembrane in sesamin group was singnificantly lighter than that in model group.(2)The TC,TG and LDL in sesamin group were significantly lower than that in model group (p＜0.01).(3)Serum intercellular adhesion molecule-1 in sesamin group was significantly lower than that in model group.Serum intercellular adhesion molecule-1 was positively associated with TC and LDL.Conclusions:(1)Sesamin effectively decreased the concentrations of TC,TG and LDL,reduced the lipid deposition area,which may relate to the anti-oxidative effection as well as the inhibition of ox-LDL synthesis. (2)Sesamin effectively decreased the serum intercellular adhesion molecule-1.Sesamin could inhibit intercellular adhesion and reduce inflammation reation.(3)Sesamin treatment was more effective on inhibiting atherosclerosis.Sesamin is potential to be one of green health products for economic primary prevention from atherosclerosis.更多还原