【作者】 唐晓东；

【导师】 张木清；

【作者基本信息】 福建农林大学 ， 作物栽培学与耕作学， 2009， 硕士

【摘要】 甘蔗花叶病是一种重要的世界性甘蔗病害,由甘蔗花叶病病毒引起。目前利用病毒基因培育抗病植株的可行性已在多种病毒、多种植物中得到了证实。本研究通过基因枪转化技术获得的转ScMV-CP基因甘蔗作为材料经国家转基因安全委员会批准,开展环境释放试验。通过多年多点的环境释放试验,对转基因无性系进行抗病性、工农艺性状、产量和品质分析。研究结果表明:1.在抗病性方面5个转SCMV-CP基因品系的抗病性明显提高,其中B48在三年四点试验中发病率仅为2.64%,表现高抗,其它四个转基因甘蔗也表现抗病,花叶病发病率低于30%,而原种发病率接近100%。通过PCR检测,经过六代种植,外源基因依然保留在转基因植株体内,说明其抗病性能稳定遗传给下一代。2.由于抗花叶病能力的提高,5个转SCMV-CP基因品系的发芽整齐、苗期生长快,株高、节间长度、甘蔗蔗糖分,出汁率都得到了大幅度提高,产量和品质性状都有明显改善,与原种相比,转基因甘蔗每公顷产量增加13.7%～93.6%,甘蔗糖份绝对值提高0.07%~1.25%,而其中以以B48表现最为优异。3.通过多年多点试验的稳定性分析,转基因甘蔗品系都表现出了较好的稳定性,其中以B48和B51表现突出,在所有工农艺性状的稳定性分析中,B48综合评价最为优异。更多还原

【Abstract】 Abstract Sugarcane mosaic disease is one of major disease to sugarcane which was cuased by the sugar cane mosaic virus and influences the growth of sugarcane seriously. Breeding resistant cultivars by genetic engineering is an assistant method in high-effective breeding of sugarcane. ScMV-CP gene was transformed to high-susceptible sugarcane cv. Badila by particle bombardment in the Key Lab of Sugarcane Eco-Physiology& Genetic Improvement, Ministry of Agriculture in 2003, and 13 ScMV-CP gene transgenic sugarcane were gained. After,5 transgenic lines were chosen for environmental release according to the agronomic performance and resistance of transgenic sugarcane.Using these transgenic sugarcanes as material, the resistant stability and agronomic performance of transgenic sugarcane were studied after environmental release; Through years of environmental release of multi-point test.The results showed as followed:Resistance to ScMV was improved significantly in CP transgenic sugarcanes.and B48 which is only in the incidence of 2.64%, the other four transgenic sugarcanes but also disease-resistant, mosaic disease incidence of less than 30%, while the incidence of the original species of close to 100%.Mosaic virus as a result of anti-power, The yield and quality traits of these transgenic sugarcanes are improved.Compared with the original species, transgenic sugarcanes ’ production increased 13.7% ~ 93.6%, Sugar to increase the absolute value of 0.07% ~ 1.25%.Through multi-year stability of multi-point analysis,B48 and B51 all have good performance.Traits through the arts, industry and agriculture for the stability analysis,The transgenic line B48 was the best line in 13 transgenic sugarcane lines, and had nice agronomic traits and no disease development after inoculation or natural infection in three years.更多还原