【作者】 魏林林；

【导师】 何昭阳；

【作者基本信息】 吉林农业大学 ， 预防兽医学， 2008， 硕士

【摘要】 黏膜免疫系统是广泛分布于呼吸道、消化道、泌尿生殖道黏膜以及一些外分泌腺体的淋巴组织,是执行局部特异性免疫功能的主要场所。黏膜免疫应答过程的分泌型免疫球蛋白A分子是黏膜免疫的主要效应因子。黏膜组织是一个巨大的外环境表面,是动物有机体与外部环境进行交流的场所。黏膜是动物有机体与外界环境的防御屏障。作为黏摸免疫系统或局部免疫系统,特别在呼吸道、消化道、泌尿生殖道等黏膜内所产生的免疫活性成份,对机体感染的防御作用越来越引起人们的重视。本文通过检测商品育肥猪的口腔、胃、十二指肠、空肠、回肠、盲肠、结肠、直肠、鼻、气管、支气管、肺、膀胱、尿液、子宫角、子宫体、子宫颈、阴道、泪液、胆汁、扁桃体等器官的γ-球蛋白、IgA、IgG、IgM的含量,反映器官黏膜抗体的分布状况。全文共分三部分,分述如下:第一部分:综述了黏膜免疫的系统及其组成。免疫应答的过程及研究进展。黏膜免疫与疾病有着密切的关联。黏膜免疫系统的研究不仅为传统的免疫学增加新内容,也为很多疾病的致病机理、病原与宿主的关系、疾病诊治及新型疫苗与预防制剂等的研究提供了思路与方法。第二部分:黏膜抗体的检测。检测方法的建立,ELISA从建立到现在,已日趋成熟。它既可以检测抗原,又可以检测抗体。本试验制备了小鼠抗猪γ-球蛋白和兔抗猪γ-球蛋白、IgA、IgG、IgM的抗体,并以辣根过氧化物酶进行标记,建立了检测猪黏膜抗体的双抗体夹心ELISA方法,并对采集的各黏膜部位(每个部位30个样品)进行了检测。第三部分:检测结果,经检测商品育肥猪不同黏膜部位的抗体,绘制出其抗体含量的动态变化规律曲线。检测结果表明,消化系统、呼吸系统、泌尿系统、生殖系统、泪液、胆汁、扁桃体黏膜部位的分泌液中均含有IgA、IgG、IgM。更多还原

【Abstract】 MIS is generally distributed over air tube,enteron,mucosa of Genitourinary,and some adenoid tissue of anacrine gland,is main place of specific immunity function. sIgA of mucosa immune response process is major effect factor of mucosa immune. Mucosa tissue is a large external environment surface,is a place where animal organism and milieu exterieur exchange each other,mucosa is defensive barrier between animal organism and milieu exterieur,as a Mucosal immunesystem or local immunesystem,especially immunological activity ingredients which is happened in air tube,enteron,Genitourinary tract and also much mucosa,for defence effect of organism infected is more and more reconstr to people.This text through the detect the different parts of commodity pig,such as oral cavity,stomach,duodenum,jejunum,ileum,appendix,colon,rectum,nose,windpipe, bronchus,lung,bladder,urine,cornu uteri,body of uterus,neck of uterus,vagina,tear fluid,bile,paristhmicγ-globulin,the content of IgA、IgG、IgM,to reflect topography of organ mucosa antibody,the whole article contain three part,as following:Part 1:overview the system and composition of Mucosal immunity,process of immune response,research advancement,mucosa immune and disease have intimate correlation,the research of Mucosal immunesystem not only increase the new content for traditionary but also offer the thinking and method of research about pathopoiesis mechanism of many disease,the relationship of etiological agent and host,disease diagnose new generation vaccine and precaution praeparatum.Part 2:the establishment of mucosa antibody detection method,sampling and detection.from the establishment of ELISA to now,already gradually mature,ELISA can detect antigen,also can detect antibody,this test prepare mouse against swineγ-globulin and rabbit against swineγ-globulin,the antibody of IgA、IgG、IgM,use horse radish peroxidase;horseradish peroxidase(HRP) to labeling.to set up the method of double-antibody sandwich ELISA,establish double-antibody sandwich ELISA to detect antibody.To detect each collective mucosa position.(each position collect 30 sample product)Part 3:detection result,through detect the antibody of the commodity pigs different parts,draw development change regular curvt of antibody content,detection result indicate:secretory juice in of digestive system,respiratory apparatus,urinary system,genital system,dacryma,bile,tonsil mucosa all generous IgA、IgG、IgM.更多还原