【作者】 沈毅；

【导师】 滕理送；

【作者基本信息】 浙江大学 ， 肿瘤学， 2009， 硕士

【摘要】 目的:研究大肠癌组织中Survivin基因与RASSF1A基因表达与大肠癌发生发展的关系。方法:利用逆转录PCR技术,测定了60例大肠癌肿瘤组织样品及20例癌旁组织中Survivin基因与RASSF1A基因的mRNA表达情况;并利用甲基化特异性PCR(MSP)技术对RASSF1A启动子区域甲基化情况进行了鉴定。结果:Survivin在大肠癌肿瘤组织中的表达率为46.67%(28/60),在正常癌旁组织中只有1例表达,其表达与肿瘤转移、分化程度及Duke分期相关(P＜0.05)。RASSF1A在大肠癌肿瘤组织中的缺失率为30%(18/60),在正常癌旁组织中均有表达,其表达与肿瘤转移、分化程度及Dukes’分期相关(P＜0.05)。但survivin与RASSF1A二者表达无相关性。RASSF1A启动子的甲基化率为45%(27/60),与肿瘤分化相关(P＜0.05),并且与RASSF1A基因的表达缺失紧密相关(P＜0.001)。结论:RASSF1A启动子甲基化是导致RASSF1A基因表达缺失的主要原因,RASSF1A和Survivn基因的异常表达在大肠癌的发生、发展中起重要作用,联合检测Survivin、RASSF1A及其甲基化有可能做为反应大肠癌进展和预后的生物学指标。更多还原

【Abstract】 Objective:To investigate the expression of Survivn and RASSF1A in colorectal cancer and its clinical significance.Methods:Reverse transcription PCR was used to detect the expression of Survivin and RASSF1A in 60 cases of tumor tissue and 20 cases of surrounding normal colon tissue. Methylation-specific PCR(MSP) was used to investigate the promotor methylation of RASSF1A.Results:We found that Survivin is expressed in 28 cases of tumor tissue but only 1 case of normal tissue,RASSF1A was lost in 18 cases of tumor tissue but no case of normal tissue.We also found 27 promotor methylation in 27 cases of tumor tissue. Expression of Survivn was associated with metastasis,tumor differention and Dukes’ classification(P＜0.05).Expression of RASSF1A was associated with metastasis, tumor differention and Dukes’ classification(P＜0.05).Promotor methylation of RASSF1A was associated with tumor differention(P＜0.05) and the expression of RASSF1A(P＜0.001).Conclusion:Poromotor methylation of RASSF1A leads to absence of RASSF1A expression.Abnormally expression of Survivin and RASSF1A contributes to genesis and development of colorectal cancer.Expression of Survivin and RASSF1A andpromotor methylation of RASSF1A may be served as biomarkers for colorectal cancer diagnosis and prognosis.更多还原