电针百会、三阴交对睡眠剥夺大鼠（SD）行为学及细胞因子的实验研究

【作者】 陈利华；

【导师】 胡幼平；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2007， 硕士

【摘要】 目的:本研究采用SD模型大鼠,探讨电针对SD大鼠的行为学及相关细胞因子的影响;采用旷场实验及酶联免疫吸附检测方法,观察针灸的抗睡眠剥夺作用。方法:健康SD大鼠30只,随机分为正常对照组(简称正常组)10只,模型对照组(简称模型组)10只,电针加模型组(简称电针组)10只三组,正常组采用大平台法造模;模型组采用小平台法造模,电针组造模同模型组,给予每日一次的电针治疗。三组每日检测一般状况的变化、体重、旷场实验并采用酶联免疫吸附法测定大鼠海马IL-1β,IL-2及TNF-a含量改变。结果:电针对SD大鼠体重有一定的升高作用,能明显改变其行为学指标,能明显降低SD大鼠海马IL-1β、IL-2及TNF-a含量。结论:电针可以改善其行为学,能明显改善SD大鼠的细胞免疫功能,这种作用可能与电针促进神经内分泌免疫学机制有关。更多还原

【Abstract】 Object:To To investigate the effects of REM1 sleep deprivation (SD) on actions and cell gene levels of Sprague-eawley rats and to investigate the efficacy,of application of Electro-acupuncture for treating sleep deprivation.Methods:Thirty healthy SD rats randomly allocated to three groups(normal-control,model(A1),Electro-treatment groups). The rats of model(A1)group and Electro-treatment group were induced in male rats by housing them on small platforms overwater,normal-control group rats was on big platforms overwater.After Electro-treatment for Electro-treatment groups,the weight、the change of normal status and Open Field Test were measured with all rats,serum levels of IL-1β, IL-2,TNF-αof all groups were measured by ABC-ELISA with sleep deprivation.Results:Electro-acupuncture treatment can improve the actions and add the weight of sleep deprivation;and Electro-acupuncture can decrease the IL-1β,IL-2 and TNF-a levels.Conclusion:Application of Electro-acupuncture can decrease the IL-1β,IL-2 and TNF-a levels.,and pro-duce a fighting effect on sleep deprivation.Immune system of rats is activated by sleep deprivation as increase of IL-1β,IL-2 and TNF-a levels.The effect may be related to promote the Nerval endocrine cytokine of Electro-acupuncture.更多还原