【作者】 谢江；

【导师】 邓俊良； 左之才；

【作者基本信息】 四川农业大学 ， 临床兽医学， 2008， 硕士

【摘要】 本试验通过血清药理学试验和病理、生理试验研究复方中药“清增康”防治断奶仔猪腹泻的作用机理,为临床用药和进一步深入研究提供依据。试验一:选用健康的28±1日龄断乳长大仔猪12头,分为空白组(基础日粮组)、中药组(添加3%“清增康”的基础日粮组)和正大料组(正大全价仔猪料,含吉他霉素),每组4头,制备试验血清。采用药敏纸片法观察猪空白组血清、含“清增康”中药组血清、正大料组血清以及中药浸剂、煎剂、空白对照(蒸馏水和生理盐水)对临床腹泻病猪粪便中分离大肠杆菌和沙门氏菌的体外抑菌效果。结果表明:蒸馏水和生理盐水对分离的大肠杆菌和沙门氏菌都无抑菌作用;含“清增康”中药组血清对大肠杆菌和沙门氏菌体处抑菌效果较中药浸剂、煎剂、空白血清有明显提高,且对沙门氏菌效果略优于正大料组血清;除沙门氏菌对中药组血清高度敏感外,大肠杆菌和沙门氏菌对中药浸剂、中药煎剂、空白组血清、正大料组血清和中药组血清均呈中度敏感。试验二:选用健康的28±1日龄断乳长大仔猪36头,按照体重和性别随机分为4组,每组9头(其中♀:4头,(?):5头),3个重复,每个重复3头。对照组(Ⅰ组)、低剂量组(Ⅱ组)、中剂量组(Ⅲ组)和高剂量组(Ⅳ组)日粮中分别添加0%、0.25%、0.5%和1%的复方中药制剂“清增康”。观察不同剂量“清增康”对断奶仔猪腹泻率、生产性能、血生化、血常规、肠道大肠杆菌数和消化器官形态的影响。结果表明:1.“清增康”可降低仔猪的腹泻率,且与添加浓度(0%～1%)呈量效关系。饲料中添加0%、0.25%、0.5%和1%“清增康”的腹泻率分别为5.95%、4.36%、3.57%和1.59%;2.饲料中添加0.25%的“清增康”可使仔猪的生产性能提高9.41%,而0.5%和1%的添加量则会降低其生产性能,且随添加量升高,其抑制生长的作用更明显,比空白组分别降低1.50%和21.42%;3.“清增康”能提高血液中白细胞和中间型细胞的数量,适当降低AST、ALT、AKP、Ur、Cre的含量;4.“清增康”能显著降低仔猪盲肠、结肠和直肠内大肠杆菌的数量;5.“清增康”能提高断奶仔猪胃、肠黏膜上皮完整性。结论:复方中药“清增康”通过增强仔猪血清抗大肠杆菌和沙门氏菌能力;提高血液中白细胞,尤其是中间型细胞的数量,适当降低血液AST、ALT、AKP等酶的含量,改善心、肝、肾功能;显著降低肠道内大肠杆菌的数量;提高胃、肠黏膜上皮组织的完整性进而达到增强机体免疫力,防治断奶仔猪腹泻的目的。更多还原

【Abstract】 The mechanism of preventing and curing diarrhoea by Chinese herbal formula "Qingzengkang" in weaned Piglets was investigated through blood serum Pharmacological test,Pathological tests and Physiological tests.Experiment 1:A total of 12 piglets weaned at 28 days were randomly assigned to 3 treatment groups,which are charge group,Chinese traditional group and west medicine group,4 per set.Piglets of every group were raised by basis daily ration,basis daily ration containing 3%"Qingzengkang" and Zhengda feed.Every piglet was sampled blood from precava to prepare blood serum.Then did the drug sensitive test to bacterium coli and salmonella in k-b method.And ascertain the bacteriostatic effect of each slip according to the size of bacteriostatic circle.Results:There is not any bacteriostatic action to colibacillus and salmonella with distilled water and normal saline.The serum of Chinese herbal formula group was better than infusion,apozema and ordinary group to colibacillus and salmonella and "Zhengda" group to salmonella.Exept salmonella was hypersensitive to serum of Chinese medicine,colibacillus and salmonella were midrange sensitive to the others.Experiment 2:A total of 36 piglets weaned at 28 days were randomly assigned to 4 treatment groups,each of which was 9.They are charge group(groupⅠ),low dose group (groupⅡ),medium dose group(groupⅢ) and high dose group(groupⅣ).Every group was 3 replicates and 3 piglets per set.Piglets of every group were raised by basis daily ration with addition of "Qingzengkang",the amount of which were 0%,0.25%,0.5%and 1%in order to study the effects to diarrhoea rates,performance and so on.Results: Diarrhoea rates can be cut down by "Qingzengkang" and each of them was 5.95%,4.36%, 3.57%and 1.59%from groupⅠto groupⅣ.With the addition amount of 0.25%,the performance of piglets could be raised by 9.41%while degrade by 1.50%and 21.42%with the amount of 0.5%and 1%.And the depressant effect was enhanced with the augment of addition amount.The quantities of WBC and intermediate cells could be increased and AST,ALT,AKP,Ur and Cre could be decreased.The quantity of colibacillus of caecum, colon and intrarectal could be decreased.The organs of digestive system could be ameliorated from morphology.In conclusion,"qingzengkang" can enhance the bacteriostasis,influence the quantities of WBC,intermediate cells,AST,ALT and so on,ameliorate and function of heart and kidney,decrease the quantity of colibacillus in intestinal tract significantly,and ameliorate organs of digestive system from morphology so as to attain the effect of boosting immunity and prevention and cure diarrhea of weaned piglets.更多还原