【作者】 闫东；

【导师】 李冀；

【作者基本信息】 黑龙江中医药大学 ， 方剂学， 2008， 硕士

【摘要】 随着人类迈入21世纪,人口老龄化问题已为国际社会普遍关注和重视。本研究从中医学理论及实验研究入手,探析衰老机理,旨在寻求有效地延缓衰老的方法。二至丸为平补肝肾之阴的代表方剂,本课题以此为研究对象,以D-半乳糖致亚急性衰老大鼠为切入点,对该方延缓衰老的作用及其机理进行了较为深入的研究。目的:探源衰老之本质,研究二至丸延缓衰老的作用及其机理。方法:立足于中医理论,依托于文献资料,探讨衰老本质;并以D-半乳糖致亚急性衰老大鼠做为实验模型,维生素E为西药对照药,对二至丸低、高剂量进行药理学研究,观察大鼠一般状态,脾脏和胸腺系数,T淋巴细胞亚群CD4~+、CD8~+、CD4~+＼CD8~+比值及细胞因子白介素—2等方面的检测,以期验证二至丸各剂量对亚急性衰老大鼠的免疫调节作用,并对其发挥作用的部分机制进行探讨。结果:(1)二至丸具有显著增加D-半乳糖致衰老大鼠脾脏和胸腺重量。(2)二至丸低、高剂量具有对T淋巴细胞亚群CD4~+百分比例、CD4~+＼CD8~+的比值显著升高的作用,而CD8~+百分比无明显变化。(3)二至丸具有对细胞因子IL-2水平极显著升高的作用。结论:二至丸为平补肝肾之阴的代表方剂,作用缓和,疗效确切。二至丸具有通过调节免疫延缓衰老作用。二至丸能使D-半乳糖致衰老大鼠外周血T淋巴细胞亚群中CD4+百分比例、CD4+/CD8+比值显著升高,CD8+百分比例下降;同时能使其外周血中细胞因子IL-2水平极显著上升,因此改善了D-半乳糖致衰老大鼠机体低下的免疫功能,纠正机体免疫系统紊乱的情况,从而为临床运用二至丸防治以免疫系统功能低下或紊乱为主要特征的病症提供了科学的实验依据。更多还原

【Abstract】 With the coming of 21^stcentury,the international community has shown general attention and deeply regards towards the aging of population Beginning with the theory of traditional Chinese medicine and experimental study,the research explores the pathogenesis of aging,aiming at seeking the effective method which can delay aging.Erzhi Pill is the typical prescription for invigorating yin of the liver and kidney, which is used as the object of research.The experimental study on Erzhi Pill was researched deeply on the action and mechanism of delaying aging on sub-acute rat induced by D-galactose.Objective:To Discuss the essence of aging and research the function and mechanism of Erzhi Pill in aspect of delaying aging.Methods:Discuss the essence of aging based on the theories of traditional Chinese medicine and modern datum.Sub-acute rat of senility made by D-galactose as experimental model,Liuwei Dihuang Pill as comparative Chinese medicine and vitamin-E as comparative western medicine,this subject carries out pharmacological research of Erzhi Pill which is classified the groups of lower、midst and high dosages in order to observe the general condition of rats.Through detecting the splenic coefficient of rats,the proportion of CD4\CD8 T-lymphocyte subset and interleukin-2,the subject verifies the immunoregulatory function of delaying aging of Erzhi Pill’s each dosage towards sub-acute rats of senility and approaches the part mechanism of action.Results:（1）Erzhi Pill has the functions of significantly increasing the splenic weight of rats.（2）The lower and high dosages of Erzhi Pill have better functions of increasing the proportion of CD4\CD8 T-lymphocyte subset with the increased proportion of CD4 and the decreased proportion of CD8.（3）Erzhi Pill has the functions of significantly increasing the interleukin-2 level of rats.Conclusions:Aging of human beings is always based on yin deficiency of the liver and kidney,so its treatment is invigorating yin of the liver and kidney firstly.Erzhi Pill is the typical prescription for invigorating yin of the liver and kidney calmly,which has a mild immunoregulatory function of delaying aging with reliable effect.Erzhi Pill have better functions of increasing the proportion of CD4\CD8 T-lymphocyte subset with the increased proportion of CD4 and the decreased proportion of CD8,and increasing the interleukin-2 level,thus improving the low immunity and rectifying the immunologic derangement of the sub-acute rats induced by D-galactose,which provides scientific experimental basis for the clinical application of Erzhi Pill on preventing the syndrome characterized by low immunity or immunologic derangement.更多还原