【作者】 陆专灵；

【导师】 罗廷荣；

【作者基本信息】 广西大学 ， 预防兽医学， 2007， 硕士

【摘要】 本实验对广西毒株狂犬病病毒L基因聚合酶活性部位进行克隆和测序,截取L基因聚合酶活性部位核苷酸和推定的氨基酸序列与国外固定毒株和类狂犬病毒株进行比较分析。结果显示广西毒株与国外固定毒株的L基因聚合酶活性部位的核苷酸和推定的氨基酸同源性分别为84.8%-87.5%和94.5%-99%,与类狂犬病病毒株的核苷酸和推定的氨基酸同源性分别为75.5%-79.2%和93.5%-97%,广西各毒株之间的核苷酸和推定的氨基酸同源性较高,分别为88.7%-99.8%和96.5%-100%。根据L基因聚合酶活性部位的核苷酸序列对广西狂犬病毒株作遗传进化分析,可以将广西毒株分成3个群,群Ⅰ:GX08、GX09、GX014、GX091、GX195、GX260、GXHX、GXLA、GXSL、GXWX;群Ⅱ:GX01、GX074、GX219、GX304、GXPX、GXBM为;群Ⅲ:GXN119。比较了L基因聚合酶活性部位氨基酸的变异情况,广西毒株L基因聚合酶活性部位的特有变异为第660位、745位和771位,其中在L基因的第745位点上,广西毒株全部为精氨酸,而在该位点的国外参考毒株为丝氨酸、亮氨酸或组氨酸;在660位点上,广西毒株中GX01、GX074、GX219、GX304、GXPX和GXBM株为缬氨酸;771位点上,GX08、GX09、GX014、GX091、GX195、GX260、GXHX、GXLA、GXSL和GXWX株为丙氨酸。比较L基因聚合酶活性部位,发现其4个基序高度保守,其中基序A保持不变;基序B、C、D只有少数氨基酸发生变异,基序C中的核心序列GDNQ在各毒株中都不变。更多还原

【Abstract】 In this study the cDNA fragments containing the L gene polymerase activity module of rabies virus isolates from Guangxi were cloned and sequenced. The nucleotide sequence of L gene activity module and their deduced amino acid sequence were compared with those of other rabies virus strains and rabies-related virus strains published previously.Comparison of the nucleotide sequence and deduced amino acid sequences of polymerase activity module of rabies virus isolates from Guangxi with that of other fixed rabies virus strains and rabies-related virus strains. The nucleotide homologies and deduced amino acid homologies between isolates from Guangxi and fixed strains were 84.8% -87.5 % and 94.5 % -99 % respectively, while Guangxi isolates and rabies-related strains were 75.5%-79.2% and 93.5%-97%, respectively. The nucleotide homologies and deduced amino acid homologies between Guangxi isolates were 88.7%-99.8% and 96.5 %-100% respectively.According to the nucleotide sequence of L gene polymerase activity module, we analyzed the homology identity of the Guangxi isolates. Three group were divided. Group I include strain GX260, GXWX, GX195, GX014, GX08, GX09, GXHX, GXLA, GXSL and GX091. Group II include strain GX074, GXPX, GX01, GXBM, GX219 and GX304. Strain GXN119 belongs to Group III.Compared the amino acid mutation of the L gene polymerase activity module, The amino acid specific mutation of Guangxi strains were position 660, 754 and 771, on the amino acid position 754 of L gene, Guangxi strains all were arginine, while serine, leucine or histidine were found at the same position of other rabies virus strains and rabies-related virus strains. On the amino acid position 660 of L gene, GX01, GX074, GX219, GX304, GXPX and GXBM strains were all Valine; and on the amino acid position 771 of L gene, GX08, GX09, GX014, GX091, GX195, GX260, GXHX, GXLA, GXSL and GXWX strains were all alanine.Compared the L gene polymerase activity module, the four motif were quite conserved, Motif A is the most conserved domain in the four motifs, the Motif B, C, and D, showed several amino acid change, the core sequence CDNQ of Motif C in all strains keep conserved.更多还原