【作者】 覃剑峰；

【导师】 杨美纯；

【作者基本信息】 广西大学 ， 作物栽培学与耕作学， 2008， 硕士

【摘要】 以青天葵（Nerviliae fordii）的组培走茎为试验材料，诱导产生青天葵组培球茎，通过对青天葵组培球茎的种植，对一些影响青天葵组培球茎的诱导形成和种植的因素进行了研究，得出以下结果：1．青天葵组培球茎的诱导以青天葵组培走茎为试验材料，分别在不同季节、走茎性状、糖浓度、大量元素浓度、NAA、6-BA、PP333等条件下对走茎进行培养，诱导出青天葵组培球茎。对在各条件下诱导青天葵组培球茎的效果进行观测和比较。实验结果表明：在培养室条件下，青天葵组培球茎的诱导效果不受季节的影响；以去掉主茎茎尖、直径约0．3cm、带侧茎的组培走茎作为诱导材料，其球茎诱导效果较好；糖浓度在40％-50％范围内球茎诱导效果较好。KNO₃能够提高结球速度和数量，但不利于了球茎个体的增大，当KNO₃浓度在2800-2600mg／L范围内时诱导效果较好；NH₄NO₃有助于促进青天葵组培球茎个体的膨大，但过量的NH₄NO₃会降低诱导球茎的数量和充实度，当NH₄NO₃浓度为1650-1980 mg/L时诱导效果较好；KH₂PO₄对青天葵组培球茎的糖分积累起着重要作用，当KH₂PO₄浓度在230-272 mg／L时，诱导效果较好；在MS培养基中附加0．5-2．5mg/L NAA，有利于提高青天葵组培球茎的形成速度，对形成球茎的数量没有影响，球茎大小均匀；在MS培养基中附加0．1-0．9mg／L6-BA时，诱导球茎速度远小于未添加6-BA的培养基，且球茎数量少，质量差，效果不好；在MS培养基中附加0．2-0．9mg／LPP333时，所诱导出的球茎形态畸形，这些球茎在种植时很难发芽。2．青天葵组培球茎的移栽从2007年1月21日开始，于每月21日将诱导出的青天葵组培球茎移栽到营养杯中。对青天葵组培球茎的生长情况进行观测。实验结果表明：在2-11月份期间种植的青天葵组培球茎均能发芽生长，其中以3-4月份期间为最佳种植时间。对移栽后青天葵组培球茎的生长情况进行观测，结果表明：种球直径越大，则其发芽速度、发芽率、产量等农艺形状表现越好。当栽培基质的“沙：土=2：1或2：3”时最适合青天葵组培球茎的种植。更多还原

【Abstract】 The tissue cultured amphitropou stem of Nerviliae fordii was used as explants in this study . Several results were gotten through by inducing the amphitropou stem into corm and cultivating the corm. The results as followed:1.The induction of Nerviliae fordii cormThe tissue cultured amphitropou stem of Nerviliae fordii was used as experimental material , the explants were cultured in different season and were cultured on different sucrose concentration different macroelements concentration, and different hormones just like NAA、6-BA、PP333. The result indicated that: the induction of the corm has no relation with the season; The amphitropou stem which removed the main stem tip, and about 0.3 cm in diameter, with the side stem as explants, the induction of the corm was better, better effect of the corm induction were appeared when the sucrose concentration at 40%-50% range ; KNO₃ in favor of to improve the induction speed and corm quantity, but too high concentration had negative effect for the individual growth, the concentration of KNO₃ at 2280-2660mg/L with good effect; NH₄NO₃ contributed to the promotion of the individual corm swelling, but excessive NH₄NO₃ will reduce corm quantity , the concentration of NH₄NO₃ at 1650 to 1980mg/L with good effect; KH₂PO₄ had positive effect for accumulation of sugar in corm, when the concentration of the KH₂PO₄ at 230-272mg/L range, the induced effects was better; In the MS medium which supplemented with 0.5 to 2.5 mg/L NAA, the inducing velocity of corm can be riase, and has no effect to the amount of corms; In the MS medium supplemented with 6 -BA concentration at the range from 0.1 to 0.9 mg / L, the induction speed was worse then the control, and corm more smaller with poor quality; The corms were abnormal and it very difficult to get germination when planted in the MS medium supplemented with PP333 the concentration at the range from 0.2 to 0.9 mg/L.2. The cultivation of tissue cultured corms of Nerviliae fordiiThe tissue cultured corms were transplanted into nutrition Cup in 21st every month from January 21, 2007. The results indicated that in the period January to November the tissue cultured corms can get bud and growth, with March to April period is the best time for planting. The results indicated that the bigger of the corm the better of the germination rate, yield and other agronomic performance. The nutrition mixture "Sand: Soil = 2:1 or 2:3,"were better for corm tissue culture plant growth.更多还原