【作者】 王丽君；

【导师】 杨克迪；

【作者基本信息】 广西大学 ， 化学工艺， 2008， 硕士

【摘要】 光轴苎叶蒟,又称十八症、歪叶子兰、小麻疙瘩、大肠风等,属胡椒科胡椒属植物,有祛风散寒、舒筋活络、消瘀散肿、镇痛之功效,可用于胃寒、经痛、闭经、风湿骨痛、跌打损伤等,主要分布在云南、海南、广西、广东、福建等华南地区。本文首先对胡椒属植物的化学成分和药理作用进行了概述,接着对光轴苎叶蒟的抗抑郁活性部位进行了筛选并对其活性部位进行了化学成分的研究,具体内容如下:1.在对光轴苎叶蒟抗抑郁活性部位筛选的研究中发现,乙酸乙酯部位是光轴苎叶蒟的抗抑郁活性部位,其抗抑郁作用与盐酸氯米帕明相当,而石油醚和水提取物无抗抑郁活性。2.对光轴苎叶蒟的乙醇提取物进行了初步的化学成分的定性分析,结果显示,光轴苎叶蒟可能含有生物碱、黄酮、甾体化合物、酚类和鞣质,不含蒽醌、香豆素和萜类内酯。3.根据药理实验结果,针对活性部位-乙酸乙酯部位进行了化学成分的分离纯化,利用溶剂法、硅胶柱层析、重结晶等分离纯化方法从光轴苎叶蒟的乙酸乙酯部位分离得到5个化合物,通过化学分析方法和现代波谱技术鉴定了它们的结构。这些化合物分别是:(2E,4E)-N-isobutyleicosadienamide(EA-Ⅰ)、β-谷甾醇(EA-Ⅱ)、1,5-diphenyl-penta-1,4-dien-3-one(EA-Ⅲ)、Cepharanone B(EA-Ⅳ)、N-(2′-hydroxy-ethyl)-N-methyl-3,4-methylene-dioxybenzamide(EA-Ⅴ)。化合物(2E,4E)-N-isobutyleicosadienamide首次从光轴苎叶蒟中分离得到,N-(2′-hydroxy-ethyl)-N-methyl-3,4-methylenedioxybenzamide为新化合物。更多还原

【Abstract】 Piper boehmeriaefolium var.tonkinense belongs to genus piper plants.The plant has effects on dispelling wind and cold,stretching bones and muscles,promoting venation, releasing ecchymosis and eliminating bump,alleviating pains.Traditionally it was used to treat stomach cold,menstrual aches and amenia,rheumatic bone-ache,and injuries from falls. It mainly distributes in South China,such as YunNan,HaiNan,GuangXi,GuangDong, FuJian.Firstly,the article summarize chemical constituents and pharmacology activities of genus piper.Then,the article screens antidepressant activity of extracts from Piper boehmeriaefolium var.tonldnense and analysis its componds of active extract,the main studies as follows:During the antidepressant activity screening of Piper boehmeriaefolium vat.tonkinense, we found ethyl acetate extract shows obvious activity,comparative with antidepressant effect of chlorimipramine.However,the extracts obtained by petroleum ether and water have no antidepressant effects.The article research main chemical constituents of methnol extract from Piper boehmeriaefolium var.tonkinense.The result shows that the plant may contain alkaloids, flavonoid,steroids,phenylic acid and tannins,except anthraquione,coumarins,terpenoids.The extract of ethyl acetate is researched,and 5 organic compounds are seperated and pured from extract of ethyl acetate by extract,column-chromatograph on silica gel and recrystallization.They are(2E,4E)-N-isobutyleicosadienamide(EA-Ⅰ),β-sistosterol(EA-Ⅱ), 1,5-diphenyl-penta-1,4-dien-3-one(EA-Ⅲ),Cepharanone B(EA-Ⅳ),N-(2’-hydroxy-ethyl)-N-methyl-3, 4-methylenedioxy-benzamide(EA-Ⅴ).Compound EA-Ⅰis reported from this plant for the first time.N-(2’-hydroxy-ethyl)-N-methyl-3,4-methylenedioxy-benzamide is a new compound.更多还原