【作者】 许今博；

【导师】 买尔旦·马合木提；

【作者基本信息】 新疆医科大学 ， 药剂学， 2007， 硕士

【摘要】 目的:研究新疆紫草提取物对果蝇寿命及对D-半乳糖致小鼠衰老模型的影响,初步确定其有效成分。方法:采用野生型黑腹果蝇为试验对象,以果蝇平均寿命和最高寿命为观察指标,研究新疆紫草脂溶性提取物(AE-Ⅰ)和水溶性提取物(AE-Ⅱ)对果蝇寿命的影响;用D-半乳糖(D-Gal)建立亚急性衰老小鼠模型,观察AE-Ⅰ和AE-Ⅱ对小鼠胸腺系数、脾脏系数,肝组织总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA),脑组织一氧化氮合成酶(NOS)、单胺氧化酶(MAO),血清尿素氮(BUN)、总抗氧化能力(T-AOC)的水平;通过理化鉴别初步分析AE-Ⅰ和AE-Ⅱ中的有效成分。结果:①AE-Ⅰ各剂量组和AE-Ⅱ中、高剂量组,均可延长雌雄果蝇平均寿命(P<0.01或P<0.05),AE-Ⅰ低、中剂量组和AE-Ⅱ中、高剂量组可延长雄果蝇最高寿命(P<0.05或P<0.01),AE-Ⅰ低、中剂量组和AE-Ⅱ高剂量组可延长雌果蝇最高寿命(P<0.05或P<0.01)。②与D-半乳糖衰老模型小鼠相比,AE-Ⅱ中、高剂量组能使降低的胸腺系数升高(P<0.05或P<0.01);AE-Ⅰ和AE-Ⅱ各剂量组,均能降低血清BUN含量(P<0.01);除AE-Ⅱ低剂量组外,AE-Ⅰ、AE-Ⅱ各剂量组均可以升高T-SOD活性(P<0.01);AE-Ⅰ中、高剂量组能使肝组织GSH-Px活性升高(P<0.05或P<0.01);AE-Ⅰ各剂量组及AE-Ⅱ高剂量组均能降低MDA含量(P<0.01或P<0.05);AE-Ⅱ各剂量组对升高的MAO、NOS活性均有降低(P<0.01)。③AE-Ⅰ的主要成分为萘醌类;AE-Ⅱ的主要成分包括多糖和酚酸类。结论:新疆紫草提取物具有延缓衰老的作用。更多还原

【Abstract】 Objective: To study the effects of Arnebia euchroma (Royle) Johnston extracts (fat-soluble extract, AE-Ⅰ; water-soluble extract, AE-Ⅱ) on life-span in drosophila melanogaster (fruit fly) and on anti-aging in subacute aging mice caused by D-galactose, and the effective components initially. Methods: The effect of AE extracts on the mean and maximum life-span of fruit flies were observed, and D-galactose-aging mice model was established by injection of 1.2% D-galactose 10ml/kg. The coefficient of thymus and spleen; blood urea nitrogen (BUN) content and total antioxide capacity (T-AOC) activity in serum; superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) content in liver tissue and monoamine peroxidase (MAO), nitricoxide synthase (NOS) activities in brain homogenate were measured. Results:①The mean of both male and female fruit flies fed on media containing AE-Ⅰall dosage and AE-Ⅱmiddle and high dosage were significantly longer than those fed on pure maize powder media(P<0.05 or P<0.01); AE-Ⅰlow and middle dosage groups and AE-Ⅱm iddle and high dosage groups could significantly prolong the maximum life-span in the male fruit flies(P<0.05 or P<0.01); AE-Ⅰlow and middle dosage groups and AE-Ⅱh igh dosage groups could significantly prolong the maximum life-span of the female fruit flies compared with the control group(P<0.05 or P<0.01).②Compared with the D-galactose-aging model group, AE-Ⅱmiddle and high dosage groups could significantly increase the thymus index (P<0.05 or P<0.01); both AE-Ⅰa nd AE-Ⅱall dosage groups could obviously reduce the content of serum BUN in mice (P<0.01); Except AE-Ⅱl ow dosage group, AE-Ⅰa nd AE-Ⅱall dosage groups could significantly increase the activity of liver homogenate T-SOD(P<0.01); AE-Ⅰmiddle and high dosage groups could strengthen the GSH-Px activities in liver homogenate (P<0.05 or P<0.01); The liver MDA levels in AE-Ⅰa ll dosage groups and AE-Ⅱhigh dosage group were all obviously decreased, which were significantly higher than that in the control group (P<0.05 or P<0.01); AE-Ⅱa ll dosage groups could obviously decrease the activities of MAO and NOS in brain tissue(P<0.01); but AE-Ⅰall dosage groups had no marked effects on the index of brain homogenate mentioned above(P>0.05). Conclusions: AE-Ⅰa nd AE-Ⅱp rolong the life-span of fruit flies and showed significant anti-aging effects in subacute aging mice caused by D-galactose.更多还原