【作者】 马燕；

【导师】 颜雨春； 韩晓兰；

【作者基本信息】 安徽医科大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:观察氟化泡沫和洗必泰漱口液对于儿童牙面菌斑内的变形链球菌的影响,探讨不同时间段细菌数变化,为临床上寻求较理想的龋病预防材料提供实验依据。方法:选择80名12岁儿童分为A、B两组,每组根据DMFT的不同分为无龋组和有龋组,两组划分的标准是:DMFT=0属无龋组,DMFT＞0属有龋组。A组使用1.23%氟化泡沫,分别测干预前、1h、24h和1周的菌斑内变形链球菌数;而B组则使用0.2%洗必泰漱口,测干预前和1周的菌斑内变形链球菌数。采集右上颌第一磨牙颊面的菌斑接种于MSA培养基进行细菌培养和菌落计数,并进行变形链球菌的形态学和生化鉴定。采用SPSS单因素方差分析和t检验,以P＜0.05为有统计学意义。结果:1.牙面菌斑内的变形链球菌数在使用氟化泡沫后1h、24h和1周均明显低于干预前水平(P＜0.05):2.使用洗必泰漱口液漱口1周变形链球菌数也明显低于干预前水平。3.1周在B组的无龋组和有龋组中检出的变形链球菌数下降了57.94%和52.91%,A组中则下降了31.86%和33.77%,B组下降比例明显大于A组。结论:氟化泡沫和洗必泰漱口液都可以明显抑制儿童牙菌斑中的变形链球菌数,但洗必泰组抑菌效果相对强于氟化泡沫组,二者都可作为儿童龋病预防的方法。更多还原

【Abstract】 Objective:To evaluate the effect of fluoride foam and chlorhexidine rinse on Streptococcus mutans in dental plaque of children and the variation of bacterial levels of MS in different times.To find better prevention of dental caries provide experimental basis for the clinicalMethods:Eighty children were divided into A and B groups.Every group was divided into caries-free(DMFT=0) and caries(DMFT＞0).A group was applicated 1.23%fluoride foam and B group was applicated 0.2%chlorhexidine rinse. Bacterial levels of streptococcus mutans were evaluated in dental plaque samples in vitro 1,24h and 1 week compared the difference with pretreatment in A group,while that were evaluated in vitro pretreatment and lweek in B group.Plaque sample from buccal surface of the right maxillary first molar were inoculated into salivarious agar(MSA) for germiculture and colony count.Streptococcus mutans were applied for morphology and biochemistry assessment.Statistical analyses were carried out with the SPSS software,the level of significance was set to P＜0.05.Results:1.Bacterial levels of streptococcus mutans were lowed significantly than pretreatment after using fluoride foam 1h and 24 h and 1 week(p＜0.05).2.Bacterial levels of streptococcus mutans were decreased significantly as well after one week compared with pretreatment in chlorhexidine rinse(p＜0.05). 3.In caries-free and caries of B group,the 57.94%and 52.91%reduction in bacterial levels of streptococcus mutans after one week was more than the 31.86%and 33.77% reduction in A group.Conclusion:Fluoride foam and chlorhexidine rinse can decrease S.mutans levels in dental plaque of children and might be useful in preventing caries lesions.The effect of bacteriostasis in the group of chlorhexidine rinse were more stronger than that in the group of fluoride foam..更多还原