【作者】 陈英之；

【导师】 李容柏； 刘丕庆；

【作者基本信息】 广西大学 ， 作物遗传育种， 2007， 硕士

【摘要】 利用微卫星（simple sequence repeat，SSR）分子标记技术，结合基于分离群体分析法（bulked-segregant analysis，BSA）的隐性群体分析法（reccesive-class analysis，RCA），对含有目的基因的直立穗亲本的TC₁F₁和F₂ 2个群体的95个直立穗个体和608个弯穗个体进行了连锁分析。获得了两个位于第9染色体上的候选连锁标记RM107和RM566。遗传距离分别为14.4cM和11.5 cM，并且位于该目的基因的两侧。为了确认这个结果，又在RM107和RM566之间选取了16个在直弯亲本间有多态性的微卫星标记RM434，RMll89，RM7048，RM3600，RM410，RM242，RM278，RM201，CYZ-4，CYZ-5，CYZ-6，CYZ-10，CYZ-11和CYZ-12进行了连锁分析。研究表明：Epl（t）位点被界定在侧翼标记CYZ-6和CYZ-11之间0.14cM的遗传区域，另外1个标记CYZ-12则与Epl（t）位点完全共分离。为了构建Epl（t）位点的物理图，把与Epl（t）基因连锁的标记都通过BIA方法着陆于上述参考序列上，并由此构建了该目的基因位点的重叠群。由参考序列可以推测Epl（t）位点被界定于侧翼标记CYZ-6和CYZ-11之间43 kb的范围内。进一步通过BIA分析，确认了在该区域内具有8个开放阅读框。本研究为目的基因Epl（t）的最终克隆奠定了很好的基础，同时也可把与目的基因Epl（t）共分离的分子标记应用干分子标记辅助育种，以获得聚合了Epl（t）基因的优良品种。更多还原

【Abstract】 To determine the chromosomal location of the Epl （t） gene rapidly, a largemapping population including 95 erect panicles and 608 normal bended onesderived from F₂ and TC₁F₁ populations were constructed.A linkage analysis using candidate SSR markers was performed in themapping population through bulked-segregant analysis （BSA） based onrecessive-class analysis （RCA）. The result indicated that two markers RM107and RM566 on the chromosome 9 were linked to the gene responsible for erectpanicle trait in the respective populations above with 14.4cM and 11.5 cM ingenetic distance.After genome-wise scanning with microsatellite markers, this gene wasmapped in the long arm of chromosome 9 and 16 polymorphic markers, RM434,RM1189, RM7048, RM3600, RM410, RM242, RM278, RM201, RM24423,RM24427. CYZ-4, CYZ-5, CYZ-6, CYZ-10, CYZ-11 and CYZ-12 identified to link the gene. The result showed that the Epl（t） locus was mapped to a 0.14cM interval flanking CYZ-6 and CYZ-11, and one markers CYZ-12co-segregated with it.To physically map the Epl（t） locus, the linked markers were landed on thereference sequence of cv. Nipponbare through BIA. A contig map correspondingto the locus was constructed based on the reference sequence aligned by theEpl（t） markers. Consequently, the Epl（t） locus was defined to interval flankingmarkers CYZ-6 and CYZ-11, where only eight ORF in the region wereidentified to a DNA fragment of 43 kb in length by BIA.The fine mapping of the crucial gene responsible for erect panicle trait inrice is very valuable in developing of indica rice varieties with erectpanicles. The erect panicle rice is a new plant type in indica rice .The new ricehas some agronomic and economic functions different from the panicle bendedrice. So the close linkage markers identified in this study would be especiallyuseful for efficient marker-assistant selection in breeding program for paniclerice and map-based gene cloning in rice.更多还原