【作者】 乌日娜；

【导师】 王纯洁；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2008， 硕士

【摘要】 蒙药“特润舒都乐”制剂是诃子、姜黄、黄柏和胡黄连等七味药组成的新蒙成药。据蒙医理论,有清热、燥黄水、祛风、消炎、消肿、调节免疫力等功效。为了研究其对乳腺炎的抗炎免疫等药理学作用,初步探索了其抗菌、免疫作用和对实验性小鼠乳腺炎抗炎免疫功能的影响以及毒理学研究,为特润舒都乐制剂临床应用提供实验依据。特润舒都乐制剂抑菌作用:牛津杯法测定15种单味蒙药对大肠杆菌抑菌圈直径,并用二倍稀释法测定特润舒都乐制剂在体外的最低抑菌浓度和最低杀菌浓度,又以大肠杆菌感染小鼠,经口灌服特润舒都乐制剂以判断其体内抗菌效果。结果表明,有8种单味蒙药对该菌有较好的抑菌作用,抑菌圈直径均大于10mm,最大达到16mm;15种单药中取7味药组成的特润舒都乐方药对大肠杆菌有一定的抑制作用,对大肠杆菌的MIC为0.25g/mL、MBC为0.5g/mL;特润舒都乐制剂显著提高大肠杆菌感染的小鼠存活率。制作大肠杆菌诱发小鼠实验性乳房炎模型,开展特润舒都乐制剂对模型小鼠血细胞检测和乳腺病理变化的影响研究,结果灌服特润舒都乐方药可先增加后减少发炎乳腺中性粒细胞浸润,及早吞噬细菌,减轻细菌感染引起的组织细胞损伤,缩短发炎时间,对大肠杆菌诱发实验性乳腺炎的乳腺有一定保护作用。特润舒都乐对小鼠免疫功能的影响:采用碳廓清方法测定对正常和模型小鼠单核-巨噬细胞功能的影响;测定对正常和模型小鼠免疫脏器指数的影响;MTT法测定对正常和模型小鼠脾脏NK细胞活性的影响;采用流式细胞术检测对正常和模型小鼠外周血淋巴细胞及其亚群的影响;采用郭氏法测定正常和模型小鼠红细胞C3b受体花环率(C3bRR)和红细胞免疫复合物花环率(ICR);结果低、高特润舒都乐制剂组(120mg/kg,240mg/kg)均显著提高正常和模型小鼠单核-巨噬细胞的吞噬指数及吞噬系数;高剂量组(240mg/kg)可提高胸腺、脾脏指数;高剂量组(240mg/kg)显著提高正常CD4+T/CD8+T比值和模型鼠CD3+T细胞、CD4+T细胞和CD4+T/CD8+T比值;高剂量(240mg/kg)显著提高正常和模型小鼠脾脏NK细胞活性;显著升高红细胞C3b受体花环率(C3bRR),调节红细胞免疫功能下降,表明特润舒都乐制剂能够增强小鼠非特异性和特异性细胞免疫功能。采用剂量递增法测定了小鼠对特润舒都乐制剂的最大耐受量,结果最大耐受量最高可达到40000mg/kg,属于基本无毒者;采用特润舒都乐膏剂涂布脱毛后的家兔皮肤法测定了对完整皮肤及受损皮肤的刺激性反应。结果显示,皮肤均未见红斑与水肿,表明该药对皮肤无刺激性。更多还原

【Abstract】 Terun-sodolol is a kind of new Mongolian medicine made of seven ingredients, such as Hezi, Jianghuang, Huangbai and Huhuanglian etc. According to the theory of the Mongolian medicine, it could antipyetic, hot and dry, diminish inflammation, detumescence, regulate the immune-ability and has some other functions. To study the anti-inflammatory and immunology mechanism in pharmocology of it on the mastitis, the medicine effect of it on the antibiosis, immunity and the immunological function on the experimental mice and the toxicology were primarily studied, which provides clinical information for its usage.The Bacteriostasis circle diameter of 15 single medicine to Escherichia coli were determined by Oxford cup law, the Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Terun-sodolol to Escherichia coli were examined in vitro by Two times dilution method. The antibacterial effect of Terun-sodolol on the mice infected by Escherichia coli was also observed after Mice were given different doses of Terun-sodolol. The results showed: 8 kinds of 15 single Mongolian medicines had significant inhibiting effects on Escherichia coli,Bacteriostasis circle diameter was bigger than 10mm, achieved 16mm most greatly. Terun-sodolol Compound prescription made of 7 single medicine of 15 single medicine above in vitro had the good bacteriostasis function, The MIC and the MBC of it for the colibacillus was 0.25g/ml and 0.5g/ml respectively;The infectious mouse survival rate was significantly advanced in taking orally the different dosages Terun-sodolol. The model of the mammitis of the mice induced by the colibacillus was made. Then the effect of it on the blood cells and the mammary gland pathological changes of the experimental mice were examined, the results showed the infiltration of the PMN in the infected mammary gland were increased at first and decreased then in oral taking medicine group, to engulf bacteria in time, decreasing of the bacteria damage of the infected tissue and shortening of the infection time, which showed certain effect on the protection of the gland from the colibacillus. The effect of Terun-sodolol on the function of the immunology: the function of mononuclear macrophage of the normal and model mice were studied through the carbon particle clearance test. The activity of the NK cells of the normal and model mice was studied with MTT method, the immune viscera index were examined, peripheral-blood lymphocytes and T-Cell subpopulations were also studied with the flow cytometry, and the C3bRR and the ICR were studied with the Guoshi test. The results showed: phagocytosis coefficient and the phagocytosis index of the mononuclear macrophage were significantly advanced in the common and model groups both of the high and low dosages groups(120mg/kg,240mg/kg). The index of the thymus and the spleen were advanced in the high dosage group (240mg/kg). The ratio of CD4+T/CD8+T of the control group and the CD3+T, CD4+T and the ratio of the CD4+T/CD8+T of the high dosage groups were significantly improved. The activity of the NK cells of the mice spleen was also improved significantly in the high dosage group. TheC3bRR was significantly advanced. These results suggest that Terun-sodolol could improve the non-specific and cell specific immune function. With the method of increasing dose the most tolerance volumue of the mice for the Terun-sodolol was determined as 40000mg/kg, which showed innocuity basically. The painting of the Terun-sodolol on the depilated skin of the rabbits showed no erythema and edema on skin, which indicated that no stimulate action for skin.更多还原