【作者】 吴晓英；

【导师】 陈卫昌；

【作者基本信息】 苏州大学 ， 消化内科， 2008， 硕士

【摘要】 目的研究KLF4基因在胃腺癌组织中的表达以及与胃腺癌临床病理参数之间的关系,以探讨KLF4基因在胃腺癌发生、发展中的作用。方法免疫组化和逆转录-聚合酶链反应(RT-PCR)分别检测50例人胃腺癌组织及相应癌旁胃粘膜组织中KLF4、Sp1蛋白表达和mRNA转录水平。结果免疫组化显示胃腺癌中KLF4阳性率为64%(32/50),强阳性率为40%(20/50),癌旁胃粘膜组织中KLF4阳性率为100%(50/50),强阳性率为72%(36/50),胃腺癌与癌旁胃粘膜组织相比明显降低(P<0.01)。胃腺癌组织中Spl阳性率为60%(30/50),强阳性率为28%(14/50),癌旁胃粘膜组织中Spl阳性率为30%(15/50),强阳性率为0%(0/50),胃腺癌与癌旁胃粘膜组织相比明显增高(P<0.01)。RT-PCR检测显示胃腺癌组织中KLF4 mRNA相对表达强度明显低于癌旁胃粘膜组织(0.565±0.324 vs 1.045±0.249, P<0.01),Sp1 mRNA相对表达强度显著高于癌旁胃粘膜组织(1.213±0.238 vs 0.684±0.294, P<0.01)。KLF4与Sp1蛋白和mRNA转录水平表达异常均与胃腺癌分化程度、淋巴结转移和TNM分期有关(P<0.05),但与患者年龄、性别、肿瘤部位、肿瘤大小和组织类型等无明显相关性(P>0.05),KLF4与Sp1 mRNA转录水平表达呈负相关(相关系数r = -0.652,P<0.05)。结论KLF4表达降低、Sp1表达增高与胃腺癌分化程度、淋巴结转移及TNM分期有关,检测KLF4和Sp1基因对预测胃腺癌进展有一定价值。更多还原

【Abstract】 Objective: To investigate the expression of KLF4 in human gastric adenocarcinoma and the correlation with clinicopathological parameter of gastric adenocarcinoma . To explore the role of KLF4 gene on the development and progression of gastric adenocarcinoma .Methods: All samples were obtained from surgically resection specimens of 50 patients , which contain primary gastric adenocarcinoma and corresponding non- cancerous gastric mucosa . The expressions of KLF4 and Sp1 protein of samples were detected by immunohistochemistry , the expressions of KLF4 and Sp1 mRNA were detected by reverse transcription-PCR(RT-PCR).Result: The positive expression rate of KLF4 protein in gastric adenocarcinoma was 64%(32/50), strong positive staining was 40%(20/50), whereas the positive expression rate of KLF4 protein in corresponding non-cancerous gastric mucosa was 100%(50/50), strong positive staining was 72%(36/50). Compared with non-cancerous gastric mucosa, the level of KLF4 protein was significantly decreased in gastric adenocarcinoma(P<0.01) . The positive expression rate of Sp1 protein in gastric adenocarcinoma was 60%(30/50), strong positive staining was 28%(14/50), whereas the positive expression rate of Sp1 protein in corresponding non- cancerous gastric mucosa was 30%(15/50), strong positive staining was 0%(0/50). Compared with non-cancerous gastric mucosa, the level of Sp1 protein was significantly increased in gastric adenocarcinoma ( P<0.01 ) . The expression of KLF4 mRNA in gastric adenocarcinoma was significantly lower than that in non-cancerous gastric mucosa(0.565±0.324 vs 1.045±0.249, P<0.01), the expression of Sp1 mRNA in gastric cancer was higher than that in non-cancerous gastric mucosa(1.213±0.238 vs 0.684±0.294, P<0.01). Both KLF4、Sp1 protein and mRNA expression levels was correlated with differentiation , lymph node metastasis and TNM stage(P <0.05), but has no correlation with other clinic parameters as age , gender , histological classification , tumor size and tumor location(P>0.05), and a significantly negative correlation between KLF4 and Sp1 mRNA expression was found(r = -0.652 , P<0.05).Conclusion: Down-regulation of KLF4 and up-regulation of Sp1 was correlated with differentiation , lymph node metastasis and TNM stage . KLF4 and Sp1 gene may be powerful indicators for prediciting the progress of gastric adenocarcinoma .更多还原