【作者】 赵丽娟；

【导师】 张茹慧；

【作者基本信息】 吉林大学 ， 口腔临床医学， 2008， 硕士

【摘要】 目的:研究人口腔鳞状细胞癌中信号转导子和转录激活子3(STAT3)蛋白的表达情况,探讨其表达与口腔鳞癌的关系及其用于口腔鳞癌基因治疗的可行性。方法:采用免疫组织化学SABC法检测42例口腔鳞癌和癌前病变组织中STAT3蛋白的表达。利用自动图像分析仪定量分析STAT3的相对含量(灰度)。结果:口腔鳞癌组织中STAT3蛋白表达水平明显高于癌前病变组织(P＜0.05),STAT3蛋白在高中低分化鳞癌组中的平均灰度值组间差异具有统计学意义(P＜0.01)。肿瘤分化程度越高STAT3蛋白表达越低。STAT3蛋白表达水平与肿瘤大小相关,T1、T2、T3间差异具有统计学意义(P＜0.01)。Ⅲ、Ⅳ期口腔鳞癌STAT3蛋白表达水平高于Ⅰ、Ⅱ期(P＜0.01)。有淋巴结转移的口腔鳞癌组织中STAT3蛋白表达明显高于无淋巴结转移者(P＜0.01)。STAT3蛋白的表达与患者的年龄、性别及肿瘤部位无明显相关(P＞0.1)。结论:STAT3蛋白的表达可能影响着口腔鳞癌的发生、发展,并与其分化程度、临床分期及淋巴结转移有关,有望成为口腔鳞癌诊断和治疗的指标。更多还原

【Abstract】 Objective:To investigate the expression of signal transducers and activators of transcription 3(STAT3)in human oral squamous cell carcinoma and to evaluate the relationship between protein level and clinicopathological parameters and the probability of the STAT3 using in the gene therapy.Methods:The immunohistochemistry SABC was used to detect the expression of the stat3 in human oral squamous cell carcinoma and precancerous lesion.The automatic image analyser was used to analyse the gray scale of the STAT3.Result:STAT3 protein level was significantly increased in squamous cell carcinoma tissue as compared with precancerous lesion(P＜0.05).There were statistical differences of the average gray scale between the different differentiated cancers of the STAT3 protein(P＜0.01).The expression of STAT3 was higher in poor-differentiated cancers than in well-differentiated cancers.The expression of STAT3 was significantly different between the T1、T2 and T3(P＜0.01).Metastatic lymphnodes overexpressed STAT3 protein as compared with non-metastatic lymphnodes.Protein level of STAT3 in the stageⅢand stageⅣcarcinomas was higher than that in stageⅠand stageⅡcarcinomas(P＜0.01).Conclusion:Overexpression of STAT3 may play an important role in the carcinogenesis、development and the clinical stage of the human oral squamous cell carcinoma and it may be one of the indexes of the diagnosis and therapy.更多还原