【作者】 张龙；

【导师】 杨成章；

【作者基本信息】 华中科技大学 ， 耳鼻咽喉科学， 2006， 硕士

【摘要】 目的本研究通过观察正常喉部黏膜和喉癌组织中趋化因子受体CXCR4和CCR7的表达情况,初步了解趋化因子受体CXCR4、CCR7与喉癌病理分化程度、临床分期及淋巴结转移之间的关系。方法应用免疫组织化学SP法对实验组30例喉癌组织标本和对照组20例正常喉部黏膜组织标本中的趋化因子受体CXCR4和CCR7的表达进行检测,并应用计算机辅助图像分析仪对表达结果进行图像分析,然后进行统计学分析。结果(1)趋化因子受体CXCR4和CCR7蛋白在大部分喉癌组织中呈强阳性表达(其阳性率分别为83.3%,86.7%),而在正常喉部黏膜组织中呈阴性或弱阳性表达(其阳性率分别为35%,30%),两组间比较有显著性差异(P<0.01) ;(2)趋化因子受体CXCR4和CCR7蛋白在低、中分化喉癌组织中的表达水平较高分化喉癌组明显升高(P<0.05),同样临床Ⅲ～Ⅳ期组较临床Ⅰ～Ⅱ期组、以及有淋巴结转移组较无淋巴结转移组,趋化因子受体CXCR4和CCR7蛋白的表达水平均明显升高(P<0.01);(3)趋化因子受体CXCR4和CCR7蛋白在喉癌组织中的表达水平两者之间有显著相关性(r = 0.837, P=0.01)。结论趋化因子受体CXCR4和CCR7在喉癌组织中的高表达,其表达水平与喉癌的病理分化程度、临床分期及淋巴结转移有关。更多还原

【Abstract】 Objective To observe the expressions of CXCR4 and CCR7 in normal laryngeal mucous membrane tissues and laryngeal carcinoma tissues and primitively clarify the relationship between CCR7 or CXCR4 and the malignity information of laryngeal carcinoma such as differentiation grade, clinical stage and lymph node metastasis.Methods The expressions of CXCR4 and CCR7 in 30 cases laryngeal carcinoma tissues(experiment group)and 20 cases normal laryngeal tissues(control group)were examined with immunohistochemistry. And qualitative analysis of light microscopy with computer-assisted image system was employed.Results Our results showed: (1) CXCR4 and CCR7 were positively expressed in most laryngeal carcinoma tissues(the positivity percentage were 83.3% and 86.7% respectively)while they were negative or low-positive in the control group(the positivity percentage were 35% and 30% respectively). There are significant differences between experiment group and the control group (P<0.01). (2) CXCR4 and CCR7 expressions in laryngeal carcinoma declined with the rise of the differentiation grade of laryngeal carcinoma(P<0.05). The levels of CXCR4 and CCR7 expressions were significantly higher in the group with lymph node metastasis than those without lymph node metastasis(P<0.01). And the same results appeared between laryngeal carcinoma groups with different clincical stages(P<0.01)(.3)The level of CXCR4 expression in laryngeal carcinoma tissues was correlated with the counterpart of CCR7(r=0.837, P=0.01).Conclusion CXCR4 and CCR7 are highly expressed in laryngeal carcinoma, and their expressions were associated with differentiation grade of laryngeal carcinoma , clinical stage and lymph node metastasis.更多还原