【作者】 邓超；

【导师】 邵增务；

【作者基本信息】 华中科技大学 ， 骨外科， 2006， 硕士

【摘要】 目的:比较p16^INK4a和p19^ARF2种抑癌基因在可切除的人骨肉瘤组织中的表达形式,探讨其在人骨肉瘤的发生、发展中的意义。方法:人骨肉瘤切除石蜡标本37例,用单克隆抗体间接免疫荧光法同时测定肿瘤组织的p16、p19蛋白的表达。结果: p16表达率56.8%（21/37）, p19表达率40.5%（15/37）;p16与p19表达无相关性;p16和p19与骨肉瘤的外科分期、病理分级、血管浸润和肺部转移均无显著相关。结论:p16、p19的下调都很可能是骨肉瘤发生发展的重要原因之一,p16和p19在骨肉瘤中表达途径不同,未发现这2种抑癌基因之间及与骨肉瘤发生发展的显著关系。更多还原

【Abstract】 Objective : To elucidate the differences in tumor genesis , progression on the expression of p19ARF and p16INK4a in human osteosarcoma.Methods : Paraffin embedded surgical specimens from 37 cases of human osteosarcoma are used to detect p19ARF and p16INK4a proteins by monoclonal indirect immunofluorescence method.Results : p16INK4a protein was detected in 21 of 37(56.8 %) cases in human osteosarcoma , p19ARF protein was detected in 15 of 37(40.5 %) cases in human osteosarcoma : no significant correlate expression of p19ARF proteins and p16INK4a protein was found in human osteosarcoma. No obvious correlations were found between the expression of p16 and p19 and the tumor staging , grading , major vascular involvement and lung metastasis in hunman osteosarcoma.Conclusion :P16 and p19 downregulation seems to be a important event in tumor genesis and progression in hunman osteosarcoma. In hunman osteosarcoma , none of these two tumor inhibiting genes seems to play important roles.更多还原