【作者】 汪星；

【导师】 赵开弘；

【作者基本信息】 华中科技大学 ， 环境科学， 2006， 硕士

【摘要】 本文研究了邻苯二甲酸二丁酯(DBP)对鱼腥藻PCC 7120生长的影响。研究结果表明,在培养基中加入少量DBP对鱼腥藻PCC 7120的生长有一定的刺激作用,当加入DBP的浓度为1 mg/mL和2 mg/mL时,与对照藻相比,鱼腥藻PCC 7120的藻密度的最大值分别高出了10 %和20 %,当DBP加入的浓度为4 mg/mL时,其藻密度低于对照值,并且随着DBP加入量的增大,鱼腥藻PCC 7120的生长逐步受到抑制,直至衰亡。通过测定叶绿素a(Chla)、藻蓝蛋白含量(c-pc)、可溶性蛋白含量、三磷酸腺酐(ATP)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、过氧化物酶(POD)、过氧化氢酶(CAT)等生长代谢指标,发现加入少量的DBP促进了鱼腥藻PCC 7120的生长;当鱼腥藻PCC 7120进入生长末期时,对照藻液开始发黄且各种生理指标呈现下降(藻蓝蛋白含量下降95 %、ATP含量下降72 %、可溶性蛋白含量下降90 %、SOD酶活性下降45 %),而加入DBP浓度为1 mg/mL和2 mg/mL的藻液仍保持绿色,藻蓝蛋白含量仅分别减少35 %和37 %,ATP含量仅分别减少12 %和7 %,SOD含量仅减少12 %和20 %,可溶性蛋白含量减少56 %和48 %。由此可见,DBP对鱼腥藻PCC 7120的生长产生了影响,延缓了其藻细胞的衰亡。而加入DBP浓度为4 mg/mL的鱼腥藻PCC 7120,由于提前进入衰亡期,使得在正常藻进入衰亡期时,其已经进入下一个生长周期,从而测得的各项生理指标比对照要高。更多还原

【Abstract】 This article dealt with the effects of Di-n-butyl phthalate on the Growth of Anabaena sp. PCC 7120.The results showed that soft stimulation effect appeared in lower concentration of DBP.The algae density was 10 % and 20 % higher than control when the concentration of DBP was 1 mg/mL and 2 mg/mL,respectively.But the algae density was lower than control when the concentration of DBP was 4 mg/mL,and the algae density became lower with more DBP.It was also indicated that the soft stimulation effect appeared in lower concentration of DBP by measuring the contents of algal Chlorophyll a,c-phycocyanin(c-pc), soluble protein,adenosine triphosphate (ATP),superoxidase (SOD),glutathione(GSH), peroxidase (POD) and catalase(CAT).Interestingly, at the end of the growth period,the control algae became yellow, and the various physiological parameters declined rapidly (C-PC declined by 95 %,ATP declined by 72 %,soluble protein declined by 90 %,SOD declined by 45 %).However,the algae which had been added to 1 mg/mL and 2 mg/mL DBP were still green,C-PC declined only by 35% and 37%,the ATP declined only by 12 % and 7 %, the SOD declined by 12 % and 19 %,although the soluble protein declined by 56 % and 48 %.It was found that DBP had evident effects on the growth of Anabaena sp. PCC 7120 and delayed the contabescence.Because of the earlier contabescence,the algae which add to 4 mg/mL DBP had been in the next growthing period and its physiological parameters were higher than the control which was in the period of contabescence.更多还原