【作者】 哈斯额尔敦；

【导师】 敖长金；

【作者基本信息】 内蒙古农业大学 ， 动物营养与饲料科学， 2007， 硕士

【摘要】 在精粗比40:60的条件下,采用单因子随机试验设计,应用体外批次培养方法,筛选了舍饲绵羊精料中的沙葱提取物(水溶性提取物,AWE和脂溶性提取无物,AFE)添加量,并研究了AWE和AFE对绵羊瘤胃体外发酵参数的影响,根据以上试验数据,选用60只健康的蒙古羯羊,采用完全随机区组试验设计分为5个组(分别为AWE组、AFE组、AWE&AFE混合组、4%沙葱组和对照组),进而研究了添加沙葱及沙葱提取物对舍饲绵羊脂肪代谢的影响。试验结果如下:1根据发酵体系的产气量、NDF降解率、NH3-N含量、BCP浓度、pH值和VFA产量变化的综合指标确定绵羊日粮中AWE适宜添加范围为:402～498mg/kg日粮;AFE适宜添加范围为:10～30mg/kg日粮。2在饲养试验中,混合组显著降低了皮下脂肪SFA的含量和C18:2/C18:3比例,增加了MUFA和PUFA的含量;混合组显著降低了肾周脂SFA的含量,增加了MUFA含量,AFE组显著增加了肾周脂MUFA的含量和C18:2/C18:3比例;AWE组显著降低了背最长肌SFA的含量,混合组显著增加了背最长肌MUFA含量和C18:2/C18:3比例,沙葱组增加了背最长肌PUFA的含量。不同的处理组对皮下脂肪、肾脂和背最长肌PUFA/SFA没有显著影响。3不同生长阶段四个添加组GLU水平均显著低于对照组;沙葱处理组可显著降低不同体重的TG水平,对CHO水平没有显著影响;AWE组、AFE组和混合组均显著降低HDL和LDL水平。4 AWE组和AFE组显著降低皮下脂肪FAS活性,混合组显著降低肾周脂FAS活性,混合组、沙葱组和AWE组可显著降低肝脏FAS活性;混合组可显著增加皮下脂肪HSL活性,混合组和沙葱组可显著增加肾脂HSL活性,混合组、AWE组、AFE组可显著增加肝脏HSL活性;四个处理组均显著降低皮下脂肪LPL活性,AFE组和混合组显著增加肾周脂和肝脏LPL活性。5 AWE组和混合组显著降低不同体重绵羊IGF-1;混合组显著增加不同体重绵羊TNF-a ;四个处理组对不同体重绵羊LEP没有显著影响,但四个处理组的LEP略低于对照组。6沙葱及其提取物对20kg体重绵羊IF和TBF没有显著影响,但可显著降低30kg体重和40kg体重绵羊IF和TBF。更多还原

【Abstract】 This study was conducted to investigate effects of different additive Allium Mongolicum Regel extract( water soluble extract, AWE and fat soluble extract, AFE ) on rumen fermentation by batch culture procedure in vitro and to select its optimum supplement for sheep. Baced on the conclusion of in vitro trail, the feeding experiment conducted with 60 healthy Mongolia wethers to studied Effects of Allium Mongolicum Regel extract on fat metabolism adopting single factors random experiment design as 5 groups(AWE group, AFE group, AWE&AFE group, 4% Allium Mongolicum Regel group and control group respectively). The results indicated that:1. It was found in vitro trail that the optimum supplement levels were 402～498mg/kg for AWE, and 10～30mg/kg for AFE respectively, baced on the parameters of gas production, pH level, NDF degradability, NH3-N, BCP and VFA concentration2. In the feeding expirement, the AWE&AFE group decreaced content of SFA and increased MUFA and PUFA contents significantly in subcutaneous fat (SF); There was significant decreace on SFA content and significant increace on MUFA content in perinephric fat (PF) when supplemented AWE&AFE as well. AWE group increased MUFA content and C18:2/C18:3 ratio in PF significantly; the AWE&AFE group also increased MUFA content and C18:2/C18:3 ratio in longissimus doris (LD) significantly. There was no significant difference on PUFA/SFA compared with control group.3. Level of GLU was decreased significantly in each supplement groups compared with control group in different growing period. Allium Mongolicum Regel group had lowest TG level and difference was significantly. There was significant decrease on levels of HDL and LDL when adding the AWE, AFE and AWE&AFE.4. The AWE group and the AFE group decreased the activity of FAS in SF and the AWE&AFE group decreased FAS activity in PF, the FAS activity in liver also decreased by Allium Mongolicum Regel group and AWE group respectively; HSL activity in SF had increased significantly by the AWE&AFE group, furthermore the AWE&AFE group and Allium Mongolicum Regel group increased HSL activity in PF sinificantly, the AWE&AFE group, AWE group and AFE group increased HSL activity in liver significantly; Each four supplement group decreased LPL activity in SF significantly, LPL activity in liver and PF were increased by AFE group and the AWE&AFE group, difference was significant. 5. There was significant decrease on level of IGF-1 in AWE group and the AWE&AFE in different body weight; The AWE&AFE ingreased level of TNF-a significantly; Each four adding group did not show significant effects on level of LEP,but the levels tended to decrease.6. There was no significant effects of Allium Mongolicum Regel and its extracts on IF and TBF of sheep as 20 kg body weight, but when the body weight growing at 30kg and 40kg it decreased IF and TBF significantly.更多还原