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臭柏无性繁殖技术
Asexual Propagation Technique of Sabina Vulgaris
【作者】 王敏;
【作者基本信息】 内蒙古农业大学 , 水土保持与荒漠化防治, 2007, 硕士
【摘要】 为了解扦插繁殖过程中各因素对臭柏插穗成活的影响,推动臭柏优良无性系的选育和大规模的育苗生产,对可能影响臭柏插穗成活和生长的外在因素进行了系统的研究。研究表明:(1)臭柏插穗生根类型不单一,愈伤组织生根型、皮部生根型、及中间生根型均有。臭柏愈伤生根型所占比例随着插穗发育程度的增加而减小。(2)实生起源的插穗优于无性起源的插穗,但硬枝Ⅲ生根情况相反。直立型母株插穗的生根性状优于匍匐型母株的插穗。灰绿直立型母株来源的插穗与黄绿直立型母株来源的插穗在生根方面无显著差异。(3)不同发育程度的插穗扦插后硬枝Ⅳ的综合效果最好,插穗生根情况呈硬枝Ⅳ>基部小侧枝>硬枝Ⅲ>硬枝Ⅱ>硬枝Ⅰ变化的趋势。硬枝插穗以10~15cm长为宜,但插穗长度对插穗生根情况无显著影响。相同发育程度的硬枝插穗随着粗度的增加生根能力呈逐渐下降的趋势。根源基多的插穗生根性状优于无根源基的插穗。插穗的留叶量在2/3以上较好。就采穗时间而言,硬枝Ⅰ、硬枝Ⅱ和硬枝Ⅲ均在6月中旬扦插生根状况较好,但硬枝Ⅳ以5月中旬最佳。(4)浸水、激素、刻伤和暗催根四种预处理(沙土基质)中,刻伤预处理对提高臭柏硬枝Ⅰ插穗的生根性状影响最显著;使用25ppm或50ppm浓度的ABT2处理对硬枝Ⅲ生根性状影响最显著。去愈处理的插穗生根情况呈现:留愈用ABT2处理>去愈不处理>去愈用ABT2处理>留愈不处理的趋势。(5)臭柏插穗扦插时强光照优于弱光照,嫩枝在较高温湿度下较好,而硬枝对温湿度的要求较嫩枝低。(6)臭柏硬枝Ⅰ在三种基质中不同水处理时间下最佳组合为原状土、浸水处理24h,硬枝Ⅲ为沙土、浸水处理6h。臭柏硬枝Ⅰ在三种基质中使用不同浓度、不同类型生根粉处理的最佳组合是混合土、50ppm的ABT1处理,硬枝Ⅲ是混合土、50ppm的ABT2处理。(7)臭柏侧枝扦插优于嫩枝扦插。混合土基质中不同起源的侧枝扦插中使用实生起源的侧枝1扦插效果最好。侧枝1和侧枝2在沙土和原状土中斜插均优于直插。
【Abstract】 In order to promote tree breeding strategy and enlarge Sabina vulgaris population, the factors affect rooting is researched systematically. The results are summarized as follows:(1) Rooting can from both of callus and roots primordiums of Sabina vulgaris. Rooting from the callus will decrease when the growths of hardwood cutting increase.(2) The hardwood from asexual mother tree is better than from sexual mother tree, but the rooting of hardwoodⅢis opposition. The hardwood from type of perpendicularity mother tree is better than from the type of grovel. There are no different between the type of two color mother tree.(3) HardwoodⅣhas best effect of rooting at the hardwood with different growth degree. The suitable hardwood remains 12cm length,6~8mm diameter and 2/3 leaf .For hardwoodⅠ、Ⅱa ndⅢthe best cutting time at the middle of June and for hardwoodⅣmiddle days of May is the best cutting time.(4) The effect of the soaking in water, hormone, wounds, lens hood on the rooting ability in hardwood cutting of Sabina vulgaris were studied respectively. The results showed that applying the wound can promote the rooting index of hardwoodⅠmostly, use 25ppm or 50ppm ABT No.2 had the best effects on the hardwoodⅢ.(5) Stronger light illumination better than weaker light illumination for hardwood rooting. High temperature and humidity benefited to roots occurrence of burgeon, but didn’t good for hardwoodⅢ.(6) The best couple for hardwoodⅠwhen soaking in water is soaking 24h then cutting in the originally soil and for hardwoodⅢis soaking 6h then cutting in the sand. Use the 50ppm ABT No.1 then cutting in the mix soil had the best effects for hardwoodⅠwhen pretreatment by hormone and use the 50ppm ABT No.2 then cutting in the mix soil had the best effects for hardwoodⅢ.(7) The rooting effect of side branches is better than burgeon. In the mix soil, the side branches1 from seedings has the best effect of rooting. The Pitch mode of cutting is better than vertical.
【Key words】 Sabina vulgaris; Cutting propagation; Mother tree; Hardwood cutting; Pretreatment; Side branches; Index of rooting;
- 【网络出版投稿人】 内蒙古农业大学 【网络出版年期】2008年 03期
- 【分类号】S791
- 【被引频次】5
- 【下载频次】203