【作者】 王艳芳；

【导师】 韩冰；

【作者基本信息】 内蒙古农业大学 ， 生物化学与分子生物学， 2007， 硕士

【摘要】 本研究以分布于内蒙古的草甸草原、典型草原、荒漠草原及荒漠上的针茅属植物为研究对象,克隆了7个物种针茅的水孔蛋白基因片段1120bp (GenBank登录号: EF061925-EF061931)。测序后用生物学软件,对其序列结构、核苷酸组成进行了初步研究,并构建了针茅属植物水孔蛋白基因进化树,同时利用PCR-SSCP分析方法研究了水孔蛋白基因的第一外显子和第一内含子的多态性。结果表明,针茅AQP基因含有3个外显子和2个内含子,外显子的大小,内含子的位置都有很高的保守性。外显子1有3种基因型(AA, BB,CC),内含子1有4种基因型(DD,EE,FF,GG),多态性只存在于物种间,而物种内均未发现多态性。针茅AQP基因所编码的蛋白质中,极性氨基酸含量高于非极性氨基酸,带正电荷的氨基酸含量高于带负电荷氨基酸。构建的三种进化树的树型基本一致,进化关系最近的是贝加尔针茅(BJE)和大针茅(DZM),支持率高于90%,然后是克氏针茅(KS),支持率也达到了70%以上,长芒草(CMC)和短花针茅(DH)在同一分枝上,支持率同样高于70%,戈壁针茅(GB)与针茅属植物其它物种形成了明显的进化分枝,进化关系与针茅生境替代性具有一致性。更多还原

【Abstract】 Seven species of Stipa L. were studied, located in meadow steppe, pink steppe, desert steppe and desert.The sequences about 1120 bp of aquaporins gene of seven species were firstly cloned and sequenced(GenBank Accession: EF061925-EF061931).The structure of sequence, the composition of nucleotide and the molecular evolution trees were analyzed by biology software. The polymorphism of exon1 and intron1 of aquaporins gene were Invested by the PCR-SSCP analysis. The results were that, AQP gene of Stipa L. had three exons and two introns, and the size and the location were high conservatism. Exon1 had three genetype (AA,BB,CC), intron1 had four genetype (DD,EE,FF,GG), and polymorphism existed in species,while no polymorphism were found within species. The composition analysis of amino acid showed that the contents of polarity amino acid was higher than that of apolarity, the contents of positive amino acid was higher than that of negative. From the molecular evolution trees constructed by different tree-building methods, three trees were similar, the evolution species of Stipa L. AQP gene were conformity with geographic distribution of Stipa L. species.更多还原