【作者】 董建江；

【导师】 甘子明；

【作者基本信息】 新疆医科大学 ， 人体解剖学与组织胚胎学， 2007， 硕士

【摘要】 目的:大鼠预先给予银杏叶片的混悬液制剂后,再给予福尔马林致痛,研究c-fos、β-内啡肽两项指标在脑内的表达变化,来观察银杏叶制剂对致痛大鼠脑内c-fos和β-内啡肽的影响。方法:取SD雌性大鼠50只,随机分为5组,模型组、药物对照组、给药组(高、中、低)三个剂量组。药物对照组给予生理盐水10天,给药组给与高中低三个剂量组的银杏叶的混悬液,每天给药两次,给药10天后,在大鼠的右脚掌给与5%的福尔马林150μl刺激,1小时后将大鼠处死,取海马和下丘脑做连续切片后,采用HE染色,在光镜下进行定位,用c-fos、β-内啡肽做免疫组化染色,对照图谱在显微镜下观察c-fos、β-内啡肽在海马区及下丘脑的阳性细胞数。结果:高剂量给药组使c-fos阳性细胞在海马的各区都减少,有统计学意义(P＜0.01),使在下丘脑内的c-fos的阳性细胞减少(P＜0.05),中、低剂量组除齿状回外,使海马区c-fos表达的阳性细胞减少(P＜0.01)。高、中剂量给药组中下丘脑室旁核、室周核、弓状核内β-内啡肽表达增加(P＜0.05)。海马的CA1CA2、CA3区β-内啡肽的表达增加主要在高、中剂量组(P＜0.05),低剂量组无差异。结论:大鼠给予银杏叶片后,炎性致痛大鼠海马区及下丘脑区的C-fos、β-内啡肽表达发生了变化,揭示了银杏叶片在疼痛大鼠的中枢神经系统对c-fos、β-内啡肽有一定的影响作用。在海马和下丘脑的三个核团C-fos表达减少,β-内啡肽表达上升。证实了银杏叶镇痛的有一定的中枢机制,为银杏叶开辟更广泛的临床应用提供了线索。同时,也揭示了c-fos与β-内啡肽之间可能存在的相互作用的关系。更多还原

【Abstract】 Objective: After rat be rended to suspl of Ginkgo biloba, through studying c-fos andβ-endorphine different expression in brain of rat being induced to pain by formalin and observing effects of Ginkgo biloba in rat brain. Method: To obtain 50 female rats and were randomly divided into 5 groups each group is 10 rats, namely model group; negative medication administration group; medication administration group (high,meddle, less) 3 doses. Negative medication administration group were rendered to Sodium Chloride, and 3 dose groups were rendered to suspl fluid of Ginkgo biloba twice each day. After 10 days rats were stimulated by 150μl of 5% formalin and put to death after 1 hours. To get hippocampus and thalamus be made serial sections, locating under light microscope by using HE staining, calculating positive cells of c-fos andβ-endorphine expression in hippocampus and thalamus with light microscope after immunohischemical technique. Result: The expression of c-fos andβ-endorphine are variety in brain between different group, expression of c-fos are decreased after rat be rendered to high dose of suspl of Ginkgo biloba in hippocampus (P<0.01), and also decreased in hypothalamus (P<0.05). Meddle and less dose suspl fluid of Ginkgo biloba cause low to the expression of c-fos in each area of hypothalamus but for dentate gyrus(.P<0.01), compare with model group and negative medication administration group. Expression ofβ-endorphine in three nucleus of hypothalamus are increased in high and meddle dose group (P<0.05) and CA1CA2、CA3 of hippocampus also be heightened (P<0.05), compare with model group and negative medication administration group. Conclusion: Ginkgo biloba effects expression of c-fos andβ-endorphine on hippocampus and thalamus to pain rat. Expression variance of c-fos andβ-endorphine confirm paregoric mechanism of Ginkgo biloba, and offer clues to more extensive clinical effects of Ginkgo biloba. Reveal interactive relation between c-fos andβ-end.更多还原