【作者】 马慧敏；

【导师】 周安国； 王之盛；

【作者基本信息】 四川农业大学 ， 动物营养与饲料加工， 2007， 硕士

【摘要】 本研究选取168只45周龄未接种大肠杆菌疫苗、体重及产蛋率相近的商品代尼克珊瑚粉产蛋鸡，采用均匀试验设计方法，设7个处理（包含一个基础对照组，一个疫苗对照组，除基础对照组外其他组均接种大肠杆菌疫苗），每个处理4个重复，每个重复6只鸡，研究了纳米硒、碘对接种大肠杆菌抗原产蛋鸡蛋中抗大肠杆菌特异性卵黄抗体和血液生化指标的影响。试验期8周。所用抗原为大肠杆菌为K88、K99、987p标准菌株。试验结果表明：1．蛋鸡日粮中添加纳米硒、碘显著提高卵黄抗体产量（P＜0.05），且纳米硒起主要作用。纳米硒、碘添加量为2.12mg／kg，4.00mg／kg时，卵黄抗体浓度达到13.48mg／ml，每毫升卵黄液抗体含量比基础对照组提高3.61mg，上升幅度为36.53％（P＜0.05），比疫苗对照组提高2.58mg，提高比例为23.63％（P＜0.05）。2．蛋鸡接种疫苗后总抗氧化能力显著下降、丙二醛水平升高（P＜0.05），补充纳米硒、碘显著提高蛋鸡总抗氧化能力（P＜0.05）、GPx活力（P＜0.01）、降低H₂O₂（P＜0.05）、MDA水平（P＜0.01）。纳米硒、碘显著提高血清T₃水平（P＜0.01）及IL-6浓度（P＜0.05），对蛋鸡血清T₄、IL-10水平无显著影响（P＞0.05）。纳米硒对抗氧化能力和IL-6的影响大于碘，碘则在提高T₃水平上占主导作用。3．接种疫苗显著降低蛋鸡产蛋性能（P＜0.05）。日粮添加纳米硒、碘显著降低疫苗刺激对蛋鸡产蛋性能的不良影响（P＜0.05）。纳米硒、碘的添加量分别为0.45mg／kg和4mg／kg时，产蛋率达91.04％，比空白对照组提高了1.48％（P＞0.05），比疫苗对照组提高26.25％（P＜0.05）；料蛋比达到2.16，比空白对照组降低了6.43％，比疫苗对照组降低了19.35％。日粮添加米硒、碘显著提高特异性卵黄抗体产量，减缓接种疫苗所引起的产蛋性能下降，纳米硒主要通过改善机体抗氧化能力和加强IL-6的分泌调节机体免疫力，进而提高卵黄抗体含量。而碘则通过提高T₃水平影响卵黄抗体含量。更多还原

【Abstract】 One hundred and sixty eight commercial Nic-T layers at 45weeks age were dividedinto seven groups（a black control, a vaccines control and five experimental groups） with 4replicates of 6 layers which there’s no significant difference in weights and egg productionand never inoculated E.coli before. A uniform design method was employed to study theeffect of nano-selenium and iodine on specific egg yolk antibody production and relateblood biochemical indicator in inoculated E.coli layers, standard bacteria of E.coli wasused in thia experiment.The results indicated that:1. E.coli inoculation increased IgY antibodies significantly（P＜0.05）.Diets with nano-seand Iodine can improve IgY antibodies significantly（P＜0.05）, nano-se was more importantalmong two facters. the supplement ofnano-se and iodine was 2.12mg/kg, 4.00mg/kg, IgYantibodies achieved 13.48 mg/kg. It was raised 3.61mg per ml egg yolk and 36.53（P＜0.05）percent against the black control group.It was raised 2.58mg per ml egg yolk and23.63（P＜0.05）percent against the inoculation control group.2. E.coli inoculation decreased T-AOC and increased MDA significantly（P＜0.05）.Diets with nano-se and iodine can improve significantly （P＜0.05）.These resultsdemonstrated that immune challenge can decreased the ability of cleaning free radical.aggravated the free-radical’s damage to somatic cell. Diets with nano-se and iodine canimprove T-AOC （P＜0.05）and GPx （P＜0.01）significantly. decreased H₂O₂ and MDA（P＜0.05）. that show that nano-se and iodine can improve the ability to clean oxygen freeradical and unsaturated hydroperoxide of layers. Diets with nano-se and iodine canimprove T₃ （P＜0.01）and IL-6（P＜0.05）significantly. But had no effect on serum T4 andIL-10 （P＞0.05）. 3. E.coli inoculation can increased IgY antibody but decreased layers performancesignificantly（P＜0.05）.Diets with nano-se and iodine can improve the performance oflayers（P＜0.05）, the supplement of nano-se and iodine was 0.45mg/kg, 4.00mg/kg, layingrates achieved 91.04%. It was raised 1.48（P＜0.05） percent against the black controlgroup.It was raised 26.25（P＜0.05）percent against the inoculation control, the suitablesupplement of nano-se and iodine for feed/egg was 0.45mg/kg, 4.00mg/kg, feed/eggachieved 2.16. It was decreased 6.43% （P＜0.05） percent against the black control group.Itwas decreased 19.35（P＜0.05）percent against the inoculation control.The effect of nano-se to antioxidation capacity and IL-6 was more important thaniodine.but iodine was the leading effect to T＿3. these described that nano-se enhanced thebody immunity through improving antioxidation capacity and strengthening secretion ofIL-6.this improved IgY antibody. iodine effected it by improving the level of T₃ in bloodserums.更多还原