【作者】 杜丽娜；

【导师】 王关林；

【作者基本信息】 辽宁师范大学 ， 植物学， 2007， 硕士

【摘要】 本论文以同属不同种的3种欧洲花楸(分别命名为R2,R3,R4)经种子诱导萌发获得的无菌试管苗为材料,将其做为外植体进行组织培养,首先摸索得到了3种欧洲花楸快速繁殖的最适培养基和R4的高效生根培养基。其次针对较难生根的R2和R3进行了生根培养,得到了R2和R3的最适生根培养基。在培养过程中重点对影响3种花楸试管苗生根的因子,例如盐浓度、外源激素、黑暗条件、蔗糖、苗龄等进行了比较研究。结果表明:3种试管苗生根均需要在低盐培养基中进行;不同种的花楸在诱导不定根形成的过程中,所需生长素种类不同。另外,苗龄对生根的影响很大,蔗糖浓度对生根的影响并不显著。针对3种花楸生根难易的不同,在试管苗生根期间,对影响生根的过氧化物酶活性和过氧化物酶同工酶电泳图谱以时间为梯度进行了跟踪测定。将测定结果与观察到的试管苗生根状态对照,发现易生根与难生根的花楸在生根过程中酶的活性变化有其各自的特点:较难生根的R2和易生根的R4其POD变化曲线呈先升高后降低的趋势,POD的启始活性相对于其整个动态曲线来看,处于一个较低的水平。而难生根的R3其POD变化曲线呈急剧下降的趋势,POD的启始活性相对于本身的动态曲线来看,处在了一个较高的水平。由此得出试管苗生根培养起始时期酶活性的差异可能是不同品种花楸生根难易差异的一个重要影响因素。通过对欧洲花楸R3和R4试管嫩茎生根过程的组织形态学观察发现,不定根起源的不同可能也是导致花楸生根难易的不同原因之一。对同一种属不同基因型的花楸试管苗生根能力的比较研究将更有利于组织培养中试管苗的生根的研究,但目前在此方面的研究工作还尚少。更多还原

【Abstract】 The European sorbus seeds of 3 species(named R2、R3、R4), which in the same genus, were used as the esperiment material to obtain the bacterialess shoots. By using these shoots as the initial explants, the stem-tip regeneration system of 4 speices and the high rooting rate medium of R4 were established.In the next place the high rooting rate medium of some difficult rooting sorbus (R2、R3 )were established. In the same time, some factors about rooting were studied to bring up the rooting rate of three species sorbus. The result showed that the rooting rates of three species were all increased in the culture medium with low salt content.Among the rooting influencing factors,the influence of shoot age was obvious on the rooting rates and the influence of sucrous was not obvious .In the physiological biochemistry target determination,the activities of POD and the electrophoretograms of POD isozymes in three different rooting ablity sorbus were investigated according to the time. The result showed that the amount of the enzyme is not the key factor to root, but the trends of them. The activities of POD of R2 and R4 are at the lower position of the activity curves but the activities of POD of R3 is at the higher position of the activity curves. It also conclued that the original activities of the enzyme in the shoot maybe more important factor, which determine the rooting ability. The paraffin method was used in anatomy research .It could be discovered that the root primordium of R3 and R4 did not exist in the same place.更多还原