【作者】 陈文祥；

【导师】 宗浩；

【作者基本信息】 四川师范大学 ， 细胞生物学， 2007， 硕士

【摘要】 从食用菌栽培下脚料废弃物中筛选分离到10株能在以纤维素（CMC-Na）为唯一碳源的筛选培养基上生长,采用刚果红鉴别培养基作进一步识别,获得透明圈较大的菌株5株。在此基础上,进行液体培养,测定酶活,得到1株酶活较高的菌株,命名为cs7912。对其形态学、细胞化学组分和生理生化特征进行研究,经初步鉴定归为链霉菌属（Streptomyces）。对其产酶条件进行研究,结果表明:接种量15%,培养基装量20%,培养基起始pH值5.2,培养温度30℃,n=125 r·min-1,正交优化后培养基组分:CMC-Na 7.5 g·L-1、滤纸2.5 g·L-1、黄豆粉10g·L-1、CaCl2·2H2O 0.3 g·L-1、MgSO4·7H2O 0.25 g·L-1、KH2PO4 4 g·L-1,液体发酵培养5d,CMCase Activity达到7.25 IU·mL-1,培养6d,滤纸酶活（Filter Paper Activity, FPA）达到2.5 IU·mL-1。菌株cs7912在最适条件下发酵所产粗酶液的最适反应pH值为5.2左右;最适反应温度为50℃左右,温度达到70℃,酶将基本失去活性;Mn²⁺,Co²⁺能较大的激活酶的活性,Zn²⁺,Cu²⁺,Ag⁺, Hg²⁺明显的抑制了酶的活性。更多还原

【Abstract】 Ten strains of microorganism which can grow in which CMC-Na as unique carbon source were isolated from offal from planting mushroom.Five colonies were got surrounded by some distinct zones of clearing in cellulose-congo red agar medium. By measuring the enzyme activity of liquid culture filtrates , we got a strain with higher cellulose activity .It was named cs7912.Its morphological , cytochemical , physiological and biochemical characters were studied. It was found that it belongs to （Streptomyces） .Its enzyme-producing conditions was studied. The results showed that 15% of inoculation,medium volume 20% ,the optimal temperature and pH are 30℃and 5.2,n=125 r·min-1,orthogonal optimal media : CMC-Na 7.5 g·L-1、Filter paper 2.5 g·L-1、Soybean powder 10 g·L-1、CaCl2·2H2O 0.3 g·L-1、MgSO4·7H2O 0.25 g·L-1、KH2PO4 4 g·L-1 ,cultivated in liquid fermentation for 5d ,the enzyme activity of CMC-Na reached 7.25 IU·mL-1 ,cultivated for 6d , FPA reached 2.5 IU·mL-1 .Properties of cellulose liquid from cs7912 cultivated in optimal conditions were investigated .The results showed that the optimal pH and temperature were 5.2 and 50℃respectively .When it reach 70℃,enzyme will lose activity nearly ; For CMCase Activity and FPA , Mn2+,Co2+ could significantly promote enzyme activity , While Zn²⁺,Cu²⁺,Ag⁺, Hg²⁺ could restrain enzyme activity .更多还原