【作者】 周鹏；

【导师】 赵仓焕；

【作者基本信息】 暨南大学 ， 中西医结合临床， 2007， 硕士

【摘要】 目的：1．观察不同时间激光穴位照射对戊巴比妥钠致眠大鼠睡眠时间的影响。2．观察激光穴位照射对对氯苯丙氨酸(PCPA)所致失眠大鼠下丘脑单胺类神经递质5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、多巴胺(DA)、去甲肾上腺素(NE)及细胞因子白细胞介素1β(IL-1β)含量的影响，探讨激光穴位照射治疗失眠的机理。3．观察激光穴位照射对失眠大鼠学习记忆能力的影响。方法：1．采用测定戊巴比妥钠致眠大鼠睡眠时间的方法，观察对照组、激光一组(照射5min)、激光二组(照射10min)大鼠的睡眠时间的变化。2．将SD大鼠24只随机分为4组：对照组、模型组、安定组、激光组，每组6只。腹腔注射PCPA建立失眠大鼠模型，氦氖激光照射神门和三阴交穴各10min，用荧光分光光度分析法和酶联免疫吸附法检测下丘脑5-HT、5-HIAA、DA、NE以及IL-1β的变化。3．用Morris水迷宫检测激光穴位照射对失眠大鼠学习记忆能力的影响。结果：1．激光组与对照组比较，戊巴比妥钠致眠大鼠的睡眠时间明显延长(P＜0.05)；激光二组与激光一组相比，明显延长戊巴比妥钠致眠大鼠的睡眠时间(P＜0.05)。2．大鼠腹腔注射PCPA后，正常的睡眠-觉醒周期消失，激光穴位照射后此周期基本恢复。3．激光组大鼠下丘脑5-HT、5-HIAA含量明显升高，与模型组比较差异非常显著(P＜0.01)，与安定组比较差异显著(P＜0.05，P＜0.01)；NE、DA含量明显降低，与模型组比较差异显著(P＜0.05，P＜0.01)，与安定组比较差异显著(P＜0.05)；IL-1β明显升高，与模型组比较差异非常显著(P＜0.01)，与安定组比较差异非常显著(P＜0.01)。4．激光穴位照射后，大鼠的逃逸潜伏期介于对照组和模型组之间，相同时点大鼠记忆能力相对提高并趋向于对照组方向，与模型组比较有显著性差异(P＜0.05)。结论：1．激光穴位照射具有镇静催眠作用。2．激光穴位照射可能是通过升高失眠大鼠下丘脑5-HT、5-HIAA及IL-1β，降低DA、NE来达到治疗失眠的目的。3．激光穴位照射可以改善失眠大鼠的学习记忆能力。更多还原

【Abstract】 Objective1. To observe the effects of different time of laser acupoint irradiation on the sleeptime of Pentobarbital sodium rats.2. To observe the effects of laser acupoint irradiation on the content of MonoamineNeurotransmitter such as N-Acetyl-5-Hydroxytryptamine(5-HT), 5-Hydroxyindole-3-acetic acid(5-HIAA), Dopamine(DA), L-Noradrenaline(NA) and Cytokines such asInterleukin-1β(IL-1β) in the hypothalamus of insomnia rats caused by DL-P-Chlorophenylalanine(PCPA) to investigate the mechanism of laser acupointirradiation treatment of insomnia.3. To observe the effects of laser acupoint irradiation on the learning and memoryability of insomnia rats.Methods1. The sleep time of Pentobarbital sodium rats was determined to observe the changeof sleep time of rats in control group, laser acupoint irradiation group I(5min), andlaser acupoint irradiation groupⅡ(10min).2. Totally 24 female SD rats were divided randomly into 4 groups with 6 rats in eachgroup: the control group, the model group, the diazepam group, the laser acupointirradiation group. The model of insomnia rats were established by PCPA abdominalinjection and the acupoints of Shenmen(HT7) and Sanyinjiao(SP6) were chosed to beirradiated by He-Ne laser. The change of 5-HT、5-HIAA、DA、NE and IL-1βin thehypothalamus of rats were observed by Fluorescence spectrophotometry andEnzyme-Linked Immunosorbent Assay.3. The influence of laser acupoint irradiation on the learning and memory ability ofinsomnia rats were tested by Morris Water Maze.Results1. Compared with the control group, the sleep time of Pentobarbital sodium rats wasobviously prolonged in two laser acupoint irradiation treatment groups(P＜0.0:5). The laser acupoint irradiation group II was better than the laser acupoint irradiation groupI(P＜0.05), obviously prolonged the sleep time of Pentobarbital sodium rats.2. After PCPA abdominal injection, the normal sleep-wakefulness cycle of ratsdisappeared; but after laser acupoint irradiation treatment, the cycle recovered.3. After laser acupoint irradiation treatment, the content of 5-HT and 5-HIAAincreased obviously in contrast with the model group(P＜0.01) and the diazepamgroup(P＜0.05, P＜0.01); while that of NE and DA decreased obviously in contrastwith the model group(P＜0.05, P＜0.01) and the diazepam group(P＜0.05). At the sametime, the content of IL-1βincreased greatly with comparison to the model group andthe diazepam group(P＜0.01).4. After laser acupoint irradiation treatment, the escape latency of rats was betweenthe model group and the control group and the memory ability of rats was greatlyimproved in the same time with comparison to the model group(P＜0.05).Conclusion1. Laser acupoint irradiation has mitigated and hypnotic effect.2. The mechanism of laser acupoint irradiation in treating insomnia is due to its goodregulation on Monoamine Neurotransmitter. It can make 5-HT、5-HIAA and IL-1βinthe hypothalamus of insomnia rats increase, and make DA、NE decrease.3. Laser acupoint irradiation can improve the learning and memory ability ofinsomnia rats.更多还原