【作者】 董祎婷；

【导师】 李爱芬；

【作者基本信息】 暨南大学 ， 水生生物学， 2007， 硕士

【摘要】 雨生红球藻（Haematococcus pluvialis）是一种单细胞淡水绿藻，在环境胁迫条件下可以合成虾青素。虾青素具有多种重要的生理功能，有广泛的应用前景。本论文以雨生红球藻（Haematococcus pluvialis CG-11）为实验藻种，研究探讨不同营养条件和环境因素对H．pluvialis CG-11生长和虾青素含量的影响。结果表明：1)添加一定量的乙酸钠对H．pluvialis CG-11的生长具有抑制作用，实验组的藻细胞生物量明显低于对照组。适宜的乙酸钠浓度有利于H．pluvialis CG-11积累虾青素，在0.5～1.0g／L时，虾青素含量是藻细胞生物量的3.22％，比对照提高了20.53％。2)低浓度的碳酸氢钠对H．pluvialis CG-11的生长没有促进作用，碳酸氢钠浓度超过1.0g／L时对藻的生长表现出明显抑制作用。适宜的碳酸氢钠浓度有利于H．pluvialis CG-11积累虾青素，当碳酸氢钠浓度在0.5～1.0 g／L时，虾青素的积累最高为生物量的3.44％，比对照提高了27.50％，此时单位体积虾青素含量最高为4.23 mg／L。3)氮、磷胁迫对H．pluvialis CG-11的生长均有抑制作用，实验组的藻细胞生物量明显低于对照组。该条件有利于H．pluvialis CG-11虾青素的积累，完全缺乏氮或磷条件下，虾青素含量最高为生物量的3.69％，比对照分别提高了21.50％、22.52％。其中，完全缺磷时，单位体积虾青素含量最高为5.86 mg／L。4)在30～35℃范围内，适宜H．pluvialis CG-11积累虾青素，虾青素含量为生物量的3.52％，比对照提高了39.69％。较低或较高的温度既不利于藻细胞生长，也不利于虾青素的积累。5)高光照抑制了H．pluvialis CG-11的生长，实验组的藻细胞生物量明显低于对照组。适宜H．pluvialis CG-11虾青素积累的光照强度为150～250μmol photons·m^-2·s^-1，虾青素含量为生物量的3.47％，比对照提高了30.93％。在光照强度200μmol photons·m^-2·s^-1条件下，单位体积虾青素含量最高为5.54 mg／L。6)提高培养液的盐度对H．pluvialis CG-11的生长具有抑制作用，实验组的藻细胞生物量明显低于对照组。低盐条件有利于H．pluvialis CG-11积累虾青素，盐度为0.25～0.75％时，虾青素含量为生物量的3.66％，比对照提高了19.55％。盐度为0.5％条件下，单位体积虾青素含量最高为6.75 mg／L。更多还原

【Abstract】 Haematococcus pluvialis is one of the unicell fresh water green microalgae. At stresscondition environment, Haematococcus pluvialis can systhesis astaxanthin. Economicmicroalgae Haemalococcus pluvialis CG-11 used as experimental material, and cultivated inBBM medium. The influence of the physical factors（temperature、illumination and salinity） andnutrition factors on cell biomass, chlorophyll a content, carotenoid content, dry weight andastaxanthin content of the spore cell has been studied, to find the optimum condition whichbenefit for spore cell’s astaxanthin accumulation. These experiments were carried out separatelyas three single factor tests. The results showed:1) The study of different sodium acetate concentration effects on astaxanthin accumulationshowed that, the concentration range from 0.5 g/L to 1.0 g/L of sodium acetate were suitable foraccumulation of astaxanthin in the spore cell of H. pluvialis CG-11. The astaxanthin contentsignificantly superior than other condition. Astaxanthin content accounted for 3.22 percent oftotal cell biomass. It was raised 20.53 percent against the control group. 1.0 g/L of sodiumacetate concentration of inhibited the growth and bring down the astaxanthin content.2) The study of different sodium bicarbonate concentration effects on astaxanthin accumulationshowed that, the concentration range from 0.5 g/L to 1.0 g/L of sodium bicarbonate were suitablefor accumulation of astaxanthin in the spore cell of H. pluvialis CG-11. The astaxanthin contentsignificantly superior than other condition. Astaxanthin content accounted for 3.44 percent oftotal cell biomass. It was raised 27.50 percent against the control group. And unit volume ofastaxanthin content accounted for 4.23 mg/L.3) The study of different nitrogen and phosphorus concentration effects on astaxanthinaccumulation showed that, nitrogen or phosphorus deficiency condition were suitable foraccumulation of astaxanthin in the spore cell of H. pluvialis CG-11. The astaxanthin contentsignificantly superior than other condition. The astaxanthin content up to 3.69 percent of totalcell biomass.. It was raised 21.50 and 22.52 percent respectively against the control group. Andunit volume of astaxanthin content reach accounted for 5.86 mg/L in phosphorus deficiency.4) The study of different temperature effects on astaxanthin accumulation showed that, thetemperature range from 30℃to 35℃were suitable for accumulation of astaxanthin in the sporecell of H. pluvialis CG-11. The astaxanthin content significantly superior than other condition.Astaxanthin content accounted for 3.52 percent of total cell biomass. It was raised 39.69 percentagainst the control group. Any level higher or lower than 30～35℃were less better for H.pluvialis CG-11 to accumulate astaxanthin. 5) The study of different illumination intensity effects on astaxanthin accumulation showed that.the illumination intensity range from 150μmol photon·m^-2·s^-1 to 250μmol photon·m^-2·s^-1 weresuitable for accumulation of astaxanthin in the spore cell of H. pluvialis CG-11. Astaxanthincontent significantly superior than other illumination intensity. Astaxanthin content accountedfor 3.47 percent of total cell biomass. It was raised 30.93 percent against the control group. Andunit volume of astaxanthin content accounted for 5.54 mg/L at 200μmol photon·m^-2·s^-1.6) The study of different salinity effects on astaxanthin accumulation showed that, the salinityrange from 0.25% to 0.75% were suitable for accumulation of astaxanthin in the spore cell of H.pluvialis CG-11. The astaxanthin content significantly superior than other condition. Astaxanthincontent accounted for 3.66 percent of total cell biomass. It was raised 19.55 percent against thecontrol group. And unit volume of astaxanthin content reach to 6.75 mg/L at 0.5% salinity.更多还原