【作者】 姜洋；

【导师】 祖元刚；

【作者基本信息】 东北林业大学 ， 植物学， 2007， 硕士

【摘要】 本实验以干旱条件下长春花根茎叶为材料，探讨长春花中与细胞壁合成相关的UDPG基因克隆和表达特征。通过对根茎叶中可溶性糖、水势的测定结果来说明干旱条件下长春花中水分缺失程度和其主要器官对于干旱胁迫反应程度的变化规律，同时结合在长春花根茎叶中UDPG基因的表达规律，揭示UDPG基因在干旱条件下影响细胞壁的合成规律和细胞壁的变化规律。研究结果如下：1、干旱胁迫下的长春花在根、茎、叶中都表现出了较正常生长条件下缺水的明显特征，说明长春花为了保持内部的代谢平衡，就要创造更多的条件从外界吸收水分。2、长春花在干旱胁迫条件下根、茎、叶器官中糖含量的变化强烈，蔗糖和葡萄糖的含量显著增加，说明干旱胁迫下细胞中糖含量的变化是植物主要应对干旱的应急机制，随着糖含量的增加，可以有效地降低植物体内的水势，以达到吸收外界水分的目的。3、通过干旱胁迫下与正常生境下长春花的根、茎、叶中UDPG基因表达量的对比，可以得出长春花在干旱胁迫下，UDPG基因表达量较正常生境下有明显增加，说明了UDPG作为糖基供体蛋白的主要功能。UDPG作为糖基相关蛋白，为干旱胁迫下长春花主要器官根、茎、叶中生物糖类的合成，细胞壁的合成以及细胞壁的变化起到了极大作用。本研究对从分子基础中找到长春花的主要抗旱手段和应用生物学手段来提高长春花的抗旱能力提供了科学研究基础，为干旱胁迫植物的应答研究机制提供了科学参考。更多还原

【Abstract】 In the present experiment, choosing Catharanthus Roseus’s root, shoot and leaf as research subject, probe into relationship of compose the cellular of Catharanthus Roseus and the character of UDPG’s clone and expression.Overpass the result of solubility-sugar, water potential in plant’s root,shoot and leaf in drought stress and the UDPG’s rule of expression,open out the composing of cellular in drought stress.It makes for the more diversification rule of cellular.1. Water potential of plants is a major index of drought stress, whose assay could indicate the degree of the soil sample suffered from drought stress, then quantify the lack of water in Catharanthus Roseus.2. The change of sugar concentration is the major emergency mechanism of plant against drought stress, the assay of which could also illuminate how the major apparatus, such as the root, shoot and leaf, respond tO the stress condition.3. As a glycosylation associated protein of secondary metabolite, UDPG could be widely utilized for the synthesis of sugar in the plant’s major apparatus; moreover it can regulate the cell wall formation and the change of cell wall in drought condition.The major emergency mechanism of plant is regulation of sugar whose glycosylation donor is UDPG, the study of which may help to figure out the resistant mechanism of Catharanthus Roseus on the molecular level. By measuring the soluble sugar and water potential, in combination with the expression of UDPG gene in root, shoot and leaf, we could confirm that the UDPG gene affects the cell wall formation in drought stress. Furthermore it might offer the possibility to work on the resistance of Catharanthus Roseus against drought in biological technology.Comparing the expression of UDPG gene in drought stress to that in normal condition, we could detect moderate up-regulation of UDPG in treatment of drought, while the increase of sucrose and glucose can be detected remarkably in the root, shoot and leaf part, which could expressly illustrate the main function of UDPG as a glycosylation donor protein.更多还原