【作者】 卓双木；

【导师】 陈建新；

【作者基本信息】 福建师范大学 ， 光学， 2007， 硕士

【摘要】 双光子显微术因其优异的特性—包含自发性的光层析、较低的光漂白与光损伤、较佳的成像对比度以及较深的成像深度，近年来在生物医学领域上，吸引了不少学者的高度兴趣。本论文旨在建立一种新双光子显微成像方法—多通道成像模式与光谱成像模式相结合的双光子显微术，并应用于人体真皮、兔子动脉壁、小鼠口腔黏膜的研究。实验结果表明，多通道成像模式与光谱成像模式相结合的双光子显微术是有潜力的工具，可用于定量显示和分析生物组织的内在成分，也可用于研究细胞的代谢情况及分析细胞的染色质结构。该技术在临床诊断和基础生物学研究领域具有广阔的应用前景。更多还原

【Abstract】 Two-photon microscopy has attracted high interests on investigation of biomedicine recently, because of its several outstanding characteristics, including intrinsic optical section, lower photon-bleaching and photo-damaging, high contrast and longer penetrating distance. In this thesis, a novel two-photon microscopy combined multichannel imaging mode with spectral imaging mode was developed to investigate biological tissues, including human dermis, rabbit arterial wall, and mouse oral mucosa. The experimental results indicate that two-photon microscopy combined multichannel imaging mode with spectral imaging mode is a potential tool to quantitatively visualize and analyze intrinsic species of tissues, and it can also be used to investigate metabolism of cells and analyze chromatin structure. The technique may prove useful to clinical diagnostics as well as to basic biological research.更多还原