正交试验初探维生素C＋E与适量游泳对S₁₈₀肿瘤模型小鼠生存期的影响

【作者】 冀鹏；

【导师】 王凤阳；

【作者基本信息】 河北师范大学 ， 运动人体科学， 2007， 硕士

【摘要】 研究目的:由于人类至今尚未真正攻克肿瘤顽疾,近年来提出带瘤生存,提高生存质量的概念。探讨如何带瘤生存、抑制肿瘤生长、提高生存质量、达到延长生存期等就成了问题的关键。本文通过给肿瘤模型小鼠补充维生素C、E并进行适量的运动干预,观察其生存期及血清TNF-α、IL-2含量及肿瘤细胞凋亡、增殖指数变化,探讨适量运动与合理补充维生素能否延长生存期以及它的可能机制,初探其运动与补充维生素剂量的合理配比,为带瘤生存,提高生存质量具有积极意义和参考价值。研究方法:本文采用正交实验设计,用L9(33)正交量表探讨,VC:0、500、1000(mg/kg)、VE:0、50、100(mg/kg)不同水平的两两配比及2种运动方案:生存天数与隔天力竭游泳运动时间的1/4倍(运动方案Ⅰ)和1/2倍(运动方案Ⅱ)动态递减式游泳运动的不同搭配对肿瘤小鼠生存期及血清中TNF-α、IL-2含量及肿瘤细胞凋亡、增殖的影响。受试对象为72只Balb/c肿瘤小鼠,然后随机分为9组,每组8只。从接种(S180肿瘤细胞)后第5至7天进行适应性游泳30min,第8天经口灌胃,给予不同剂量配比的VC、VE,并进行游泳运动训练,第40天每组各处死3只小鼠,用放免法测定小鼠血清中TNF-α和IL-2含量,用流式细胞仪检测肿瘤细胞凋亡和增殖指数。其它小鼠继续干预至第48天,观察生存期。实验结果:1、最长生存期的配方是运动方案Ⅱ+VC500mg/kg+VE50mg/kg。2、产生TNF-α、IL-2量最多的配方是运动方案Ⅰ+VE100mg/kg+VC1000mg/kg。3、促使肿瘤细胞凋亡指数最高的最佳配方是运动方案Ⅰ+VE50mg/kg+VC0mg/kg。4、促使肿瘤细胞增殖指数最高的最佳配方是VC0mg/kg +VE100mg/kg +不运动。结论:1、合理运动与VC、VE联合使用能够延长肿瘤小鼠的生存期。2、本实验提示适量运动的作用相对最为重要。3、VC、VE、运动三者联合抗肿瘤的作用,可能是升高了血液中的TNF-α、IL-2而发挥作用。4、三者的联合作用促进了肿瘤细胞的凋亡、抑制了肿瘤细胞的增殖,这可能对延长生存期起到积极的作用。更多还原

【Abstract】 Purpose:The tumor is one of the most deathful disease that threaten mankind’s health . People have been concentrated on the research that conquer tumor for a long time, but till now none of a valid method has been found. So in recent years a concept that to be lived with tumor was put forward, which means that to have a high quality life and to prolong Tumor sufferer’s life. So how to raise the quality life and how to prolong the tumor sufferer’s life has been the focus of the problem .to get the best combination plan of exercise and vitamin supplying. In this article the author supply vitamin C,E to mice of S-180 tumor-model and make them swimming, the author observe carefully mice’s lifespan and the change in the content of TNF-α、IL-2,in order to explore weather exercise and vitamin supplying can prolong mice’s lifespan and its possible mechanism.Methods:Orthogonal design (L9(33)) was used to observe the effects on mice’s lifespan and the change of the content of TNF-α、IL-2 in blood serum ,of the different combination plans of vitamin C , vitamin E:VC:0、500、1000(mg/kg)、VE:0、50、100(mg/kg)and two exercise plans, which are decreased successively in time .planⅠ:quarter of the time of exhaustive swimming every other day and days of life、Ⅱ: half of the time of exhaustive swimming every other day and days of life.Firt,72 tumor mice (Balb/c) were divided into 9 groups randomly ,8 mice each group,5 to 7 days later after inoculation, the author make the mice swimming as a adaptable training ,and on the 8th day the mice were oral administered by different dose combination of vitamin C , vitamin E and engaged in swimming exercise. On the 40th day 3 mice were executed each group, the content of TNF-α、IL-2 in blood serum in the method of radioimmunoassay(RIA) was measured and the tumor cell apoptosis index and proliferation index was detected by flow cytometry. Other mice were left to go on carrying experiment ,observe their lifespan.Results:1、combination plan of exerciseⅡ,VC500mg/kg and VE50mg/kg was the best on the longest lifespan.2、combination plan of exerciseⅠ,VE100 mg/kg and VC50mg/kg was the best on the most content of TNF-α、IL-2.3、combination plan of exerciseⅠ, VE50mg/kg and VC0mg/kg was the best on the promoting the cancer cell apoptosis.4、combination plan of VC0mg/kg and VE100mg/kg no swim was the best on the promoting the cancer cell proliferation.Conclusion:1、the combination effects of appropriate swimming , supplying vitamin C and vitamin E can prolong the lifespan of tumor mice.2、the article pointed out that proper exercise was the most important effect relatively.3、the mechanism of combination of vitamin C and vitamin E and appropriate swimming on tumor maybe that they increase the content of TNF-α、IL-2 in blood serum.4、combination of vitamin C and vitamin E and appropriate swimming can promote cancer cell apoptosis and restrain cell proliferation, which could prolong life of the mice.更多还原