中药复方对大鼠酒精性肝损伤的治疗作用

【作者】 张琦；

【导师】 张大方；

【作者基本信息】 长春中医药大学 ， 药学， 2007， 硕士

【摘要】 目的：探索中药复方组方的药效物质。通过对三个临床验方(中药复方1，2，3)的研究，从中药复方1，2，3中筛选出对治疗酒精性肝损伤效果较好的复方，并对其进行深入研究，包括其组方的特点和对酒精性肝损伤治疗的作用机理进行探讨，为临床用药提供依据。方法：初筛实验采用白酒给昆明种小鼠灌胃8周造成酒精性肝损伤模型，药物治疗4周后进行肝脏组织病理学检查以及脏器指数的观察。对治疗效果较好的复方进行深入的研究。采用传统的水煎剂和水煎醇沉的方法对其有效的成分进行研究。采用白酒灌胃SD大鼠8周造成酒精性肝损伤模型，药物治疗4周后进行肝脏组织病理学检查、脏器指数观察、以及取血用全自动生化分析仪检测血清肝功能酶TG、AST、ALT、GGT的变化，来研究中药复方的药效和有效的部位。通过对其全血中乙醇含量的测定、GST、NO和NOS、TNF测定、硒元素含量的测定初步探讨其对酒精性肝损伤的作用机理。结果：初筛实验显示，中药复方1．2．3均可以通过改善酒精性肝组织的形态变化，但中药复方1的水煎液对治疗酒精性肝损伤模型的小鼠比中药复方2和中药复方3的疗效好。中药复方1的水煎液，水煎醇沉液，及水煎醇沉的上清液对酒精性肝损伤大鼠血清中的TG、AST、ALT、GGT的含量均有降低的作用，但中药复方1的水煎液的疗效比中药复方1的水煎醇沉液和水煎醇沉的上清液的作用显著。结论：中药复方1的水煎液对大鼠酒精性肝损伤有明显的治疗作用。更多还原

【Abstract】 Objective: Exploration Chinese native medicine compound prescription group side drug efficacy material.Through to three clinical proven prescriptions (Chinese native medicine compound prescription 1.2,3) the research, in 1,2,3 screens from the Chinese native medicine compound prescription to treats the alcoholic liver damage effect good compound prescription, and conducts the thorough research to it, carries on the discussion including its group of side characteristic and to the alcoholic liver damage treatment action mechanism, provides the basis for the clinical medication.Method: Initially sieves the experiment to use the white liquor to plant the mouse for Kunming to fill the stomach 8 weeks to create the alcoholic liver damage model, after the medicine treats 4 weeks to carry on the liver organization pathology inspection as well as the internal organs index observation.Conducts the thorough research to the treatment result good compound prescription.Uses the method which traditional the water decoctum and Shui Jianchun sinks to its effective ingredient to conduct the research.Uses the white liquor to fill the stomach SD big mouse 8 weeks to create the alcoholic liver damage model, after the medicine treats 4 weeks to carry on the liver organization pathology inspection, the internal organs index observation, as well as draws blood with completely automatic biochemistry analyzer examination blood serum liver function enzyme TG, AST, ALT, the GGT change, studies the Chinese native medicine compound prescription the drug efficacy and the effective spot.Through to its whole blood in the ethyl alcohol content determination, GST, NO and NOS, the TNF determination, the selenium element content determination discusses it initially the action mechanism which damages to the alcoholic liver.Result: Initially sieves the experiment to demonstrate that, the Chinese native medicine compound prescription 1.2.3 may the shape change which organizes through the improvement alcoholic liver, but the Chinese native medicine compound prescription 1 water fries the fluid to the mouse which treats the alcoholic liver damage model the Chinese native medicine compound prescription 2 and the Chinese native medicine compound prescription 3 curative effects is better than.The Chinese native medicine compound prescription 1 water fries the fluid, Shui Jianchun sinks the fluid, and Shui Jianchun sinks the supernate damages the function to the alcoholic liver which in the big mouse blood serum TG, AST, ALT, the GGT content has reduces, but the Chinese native medicine compound prescription 1 water fries the fluid the curative effect to fry the Chinese native medicine compound prescription 1 water sinks the supernate function which the fluid and Shui Jianchun sinks to be more remarkable than mellowly.Conclusion: The Chinese native medicine compound prescription 1 water fries the fluid to have the obvious treatment function to the big mouse alcoholic liver damage.更多还原