【作者】 徐静；

【导师】 李疆； 李海真；

【作者基本信息】 新疆农业大学 ， 果树学， 2007， 硕士

【摘要】 以15个基因型的西葫芦品种为试材,进行未授粉子房或胚珠离体培养。试验结果表明:诱导的结果因基因型及培养基而异;黑暗热激对启动雌核发育有积极的作用,且以35℃热激5天效果最佳;对影响胚状体发育因子的分析表明:2,4-D对西葫芦胚状体发生是重要的,但不是必不可少的;以4mg/L作用7天诱导效果最佳。氨硝态比例对胚状体的发生有影响,加4mg/L的2,4-D时,氨态氮和硝态氮的比例以1:1为好;不加的比例为2诱导效果最好。以1个基因型品种为试材,进行了辐射花粉授粉离体培养。结果表明:辐射剂量和接种方式都对植株再生率有极显著的影响。总体上,100 Gy效果好于其它剂量。从接种方式上,以去壳的培养再生率最高。对胚囊植株的倍性鉴定方法进行比较,流式细胞仪方便准确,但费用较高,气孔保卫细胞叶绿记数是初步鉴定倍性的有效手段。形态学观察与胚胎学结果表明:胚囊再生植株是经胚状体途径产生,胚状体来源于胚囊内的成员,不是来源于内外珠被。本研究建立了一套简便实用、易于操作、移栽成活率较高的再生植株试管苗驯化、移栽的技术程序。共获得1200多株再生胚囊植株(R0),定植786株,R0代植株性状观察表明:有叶片、茎蔓和瓜形与瓜色的分离,四倍体材料具有巨大性。得种子120余份,现已播种于田间。更多还原

【Abstract】 Embryo sac plants (R0) via in vitro unpollinated ovary or ovules culture were abtained from 15 genotype squash (Cucurbita pepo L.) material The results showed that inductivity were influencedd by genotyp and medium.Hot stock in dark had an positive role, the best treatment was at 35℃for 5 days.Among the factor affected embryogenesis , 2,4-D was important ,while it was not absolutely necessarily. It was the best for treat by 4mg/L 2,4-D for 7 days . The ratio of NH4+ / NO3- also affected embryogenesis .It was better for ratio 1:1 when added 4mg/L 2,4-D ,while the best ratio was 2:1 without 2,4-D.One genotype material was used for radiated pollination in vitro culture .The results showed that the ratio of regenerated plants was strongly influenced by radiation dose and inoculation method. In sum the best dose was 100 Gy and the best inoculation method is without hull .Comparision to different ploidy indentification method for regenerated plants , flow cotometry for identification of ploidy is convenienct and accurate , but it is expensive. Chloroplast counting in guard cells was an effective tool.Morphologic observation and embryology results showed that the regenerated Embryo sac plants was via embryogenesis. Embryoid origined from Embryo sac .A acclimatizing and transplanting technique for the tube plantlet was successfully established.It is an simple,operater easily, practiceal and high survival rate for transplanting of the regenerated plants.More than 1200 regenerated Embryo sac plants (R0) were abtained and 784 were planted. R0 plant showed separation of leaf, vine, melon shape and colour.Tetraploid plant were huge. The seeds over 120 line of R0 are being planted in fields.更多还原