【作者】 于建平；

【导师】 张春庆；

【作者基本信息】 山东农业大学 ， 作物遗传育种， 2006， 硕士

【摘要】 玉米自交系遗传多样性分析是种质创新及品种选育的重要基础,是合理选配亲本,构建玉米杂种优势群,预测杂种优势的依据。本研究利用AU-PAGE技术对85个玉米自交系电泳谱带进行了分析。建立了玉米AU-PAGE蛋白质谱带的命名体系,设定了对照自交系上、中、下部三条谱带为参照谱带,三条参照谱带划分的各区域谱带的相对迁移率的计算公式分别是:Rm= Qi(x)、Rm=[1- li(x)] Qi(x)+ li(x) Ri(x)、Rm=[1- hi(x)] Ri(x)+hi(x) Si(x)、Rm= Si(x);通过分析发现不同谱带的多态性的差异,中部区域谱带多态性最高,下部次之,上部最差;通过UPGMA聚类把参试的85个自交系区分为不同的五大类:第Ⅰ类为P78599选系群,包括178等共8个自交系;第Ⅱ类为DOM群,包括塘四平头和旅大红骨两个亚群,塘四平头亚群包括黄早4等共25个自交系,旅大红骨亚群包括丹340等共3个自交系;第Ⅲ类为兰卡斯特群,包括Mo17等共13个自交系;第Ⅳ类为瑞德群,包括478等共18个自交系;第Ⅴ类为其它种质,包括107等共18个自交系,进一步验证了AU-PAGE技术在种质评价中的应用价值及可行性;建立了85个自交系的指纹数据库及蛋白指纹图谱。本研究能够为玉米种质评价、类群划分及品种保护提供技术支持。更多还原

【Abstract】 The genetic diversities of maize inbreds are the important foundation of germplasm invention and breeding variety. And the genetic diversities of maize inbreds are also the basis of selecting matched parents, constricting heterotic groups,evaluating heterosis。The electrophoretic bands of 85 maize inbreds were analyzed with AU-PAGE technique. The naming system of the protein bands was established, basis on standard bands from upside,middle and lower bands of Huangzao4 inbreds. The relative mobility rate formula of the bands in every regeion according to three standard bands were Rm=[1- li(x)]Qi(x)+ li(x) Ri(x), Rm=[1- hi(x)] Ri(x)+hi(x) Si(x)、Rm= Si(x) respectively. It showed that different bands existed the polymorphic difference. Ploymorphism of the bands in middle region was more than in upside region. Polymorphism of the bands in lower region was lower. 85 inbreds were classfied into 5 groups with UPGMA. The first group included 8 inbreds from hybrid p78599,178 included. The second group was DOM, including Sipingtou and Ludohonggu. The Sipingtou included Huangzao4 and other 24 inbreds. Ludonggu included 3inbreds, including Dan 340. The third group was Lancuster which included Mo17 and other 17 inbreds. The forth group was Reid which included 478 and other 17inbreds.The last group was others. The feasibility and application value of AU-PAGE technique in evaluating germplasm was further confirmed by UPGMA cluster analysis. In this study, the fingerprining database and the protein fingerprints of 85 maize inbreds were establised.The resultes above could give powerful technical support in evaluating germplasm, classfing groups and varity protection to maize.更多还原