【作者】 陈爱国；

【导师】 颜军；

【作者基本信息】 扬州大学 ， 运动人体科学， 2006， 硕士

【摘要】 目的：探讨中等负荷运动训练对心理应激大鼠淋巴细胞凋亡及其调控基因的影响，以探讨心理应激、体育运动及其它们交互作用对机体的影响，为进一步研究体育锻炼作为一种应对心理应激的方式提供实验和理论依据。方法：健康的雄性SD大鼠32只，随机分为4组，每组8只，即对照组、心理应激组、运动组和运动+心理应激组。运动方式为无负重游泳，每天1次，每次60分钟，共10周；心理应激则采用噪音诱发的方式，在实验的后3周，每天1次，每次120分钟，持续3周。实验后测定各组大鼠开场行为、外周血中氧自由基MDA浓度、SOD活性、淋巴细胞凋亡及其相关调控基因表达的变化情况。结果：3周的噪音刺激所引发的心理应激可对大鼠的开场行为产生显著的影响，同时可诱发大鼠体内产生大量的氧自由基，也导致大鼠外周血淋巴细胞凋亡显著增加，且其发生与相关调控基因的表达一致；10周的中等负荷的运动训练可降低大鼠体内自由基MDA的浓度，提高SOD的活性，也可以抑制大鼠外周血淋巴细胞的凋亡，且其发生与相关调控基因的表达一致；中等负荷运动训练可拮抗心理应激诱发的大鼠淋巴细胞凋亡，且其发生与相关基因表达的变化一致。结论：中等负荷运动训练拮抗心理应激导致的大鼠淋巴细胞凋亡及其相关基因表达的变化的机制可能是，中等负荷的运动训练通过降低心理应激对机体造成氧化应激损伤的反应强度，影响相关凋亡调控基因表达，从而降低大鼠淋巴细胞的凋亡。更多还原

【Abstract】 Objective: This paper analyzes the effect of moderate exercise on peripheral bloodLymphocytes apoptosis and Bax, Bcl-2 Gene expression in the psychological stress rats.Methods: Thirty two health male SD rats were divided into four groups. As follows:one as controlled group (C, n=8), one only got psychological stress (P, n=8), onereceived swimming training for 60mins (S, n=8), one received swimming training for60mins and psychological stress (PS, n=8). The Open-field test, SOD activity and theMDA content in peripheral blood of SD rats, and the Lymphocytes apoptosis and Bax,Bcl-2 Gene expression in peripheral blood of SD rats were determined.Results: Compared with C group, the numbers of crossing by P、S and PS group weresignificantly higher; the numbers of rearing by P、S and PS group were lower than Cgroup. Compared with C group, the SOD activity in P and S group were significantlyhigher, the SOD activity in PS were lower; the MDA concentration in P group weresignificantly increased, while the MDA concentration in S group were significantlydecreased, however, the MDA concentration in PS group were lower but not significantly.Compared with C group, the apoptosis in peripheral blood Lymphocytes (PBL) of ratsincreased significantly in P group and decreased significantly in S group; the apoptosis ofPS group in peripheral blood Lymphocytes(PBL) of rats decreased significantlyCompared with P group; moreover they consist with the expression of the relatedregulatory genes. The moderate exercise maybe brings down the intensity ofoxygen-stress reaction to depress the effect on peripheral blood Lymphocytes apoptosisand Bax, Bcl-2 Gene expression in the psychological stress rats.Conclusions: One of possible mechanism is that the moderate exercise maybe bringsdown the intensity of oxygen-stress reaction to depress the effect on peripheral bloodLymphocytes apoptosis in the psychological stress rats.更多还原