【作者】 郭晓静；

【导师】 宋晓平；

【作者基本信息】 西北农林科技大学 ， 临床兽医学， 2007， 硕士

【摘要】 紫杉醇对许多癌症疗效确切,但其药源一直难以解决。本研究通过对南方红豆杉的内生真菌进行分离鉴定,取得了以下结果:1内生真菌的分离鉴定:从南方红豆杉中分离出63株内生真菌,对其中24株内生真菌进行了分类鉴定,分属于19个属种:真子囊菌纲的2个属2株;无毛毛壳属和球毛壳菌各1株,无毛毛壳属1株为一新发现种;半知菌纲的12个属种22株,包括枝孢霉属3株,头孢霉属、青霉属、单端孢属曲霉属、镰孢属、单链孢属各2株,链格孢属,大茎点霉属,灰葡萄孢菌,拟青霉属各1株,未产生孢子的3株,归为无孢类群。在总分离内生真菌中,半知菌纲占91.67%,是绝对优势株。2产紫杉醇内生真菌的筛选:分离菌中的14号菌(Botrytis cinerea.)、16号菌(Mycelia sterlia)及19号菌(Mycelia sterlia)的培养液,经浓缩萃取,薄层层析检测,Rf值分别为0.404、0.404、0.401,与紫杉醇标准品Rf值一致,为南方红豆杉产紫杉醇内生真菌。更多还原

【Abstract】 Taoxl is a new kind of anti-cancer medicine having assured effect,but its nature source are very difficult to resolve. By isolation of the endophytic fungi from Taxus chinensis var maire, this research obtains following results:1 Isolation and identification of endophytic fungi: Sixty-three strains endophytic fungi were isolated and obtained from T. chinensis var. mairei, According to their morphological characterization, of which 24 strains belonged to 19 genera(species). There were 2 strains in genera of Ascomycotina:1 strain of Achaetomium sp. and 1 strain of Chaetomium globosum.; 22 strains in 17 genera of Deutermycotina: 3 strains of Cladosporium sp.; 2 strains of Ceophalosporium sp., 2 strains of Penicillium sp., 2 strains of Trichothecium sp., 2 strains of Asqergillus sp., 2 strains of Fusariumsp sp.and 2 strains of Monocillium sp.; 1 strain of Alternaria sp., 1 strain of Macrophoma sp., 1 strain of Botrytis cinerea and 1 strain of Paecilomyces sp.; and 3 strain of Mycelia sterlia. This Achaetomium sp. strain was a new species.2 Identification of the endophytic fungi : In the isolated endophytic fungi the 1 strain of Botrytis cinerea and the 2 strains of Mycelia sterlia , these concentration of strains were purificated which the Rf on thin layer chromatography(TLC)of the extracts from fermentation liquid were 0.404, 0.404, 0.401, respectively,that closed to that of sdanderd taxol. So these three strains were the taxol-producing endophytic fungi.更多还原