【作者】 黄祥英；

【导师】 陈荣； 庄惠如；

【作者基本信息】 福建师范大学 ， 光学， 2007， 硕士

【摘要】 本文介绍了微藻养殖中所面临的问题和解决的方法及激光技术在该领域应用概况。文中以一种重要的饵料微藻——亚心形扁藻为研究对象，利用Nd：YAP激光辐照亚心形扁藻，确定诱变扁藻的最佳激光剂量包括最佳功率与时间组合。同时借助紫外可见红外分光光度计、激光共焦扫描显微镜、透射电子显微镜等现代光电技术手段，研究激光辐照亚心形扁藻产生的生物学效应。通过测定经激光辐照后亚心形扁藻的各项生理指标，筛选出两株优质藻株。本研究为激光技术在微藻诱变领域的进一步应用，提供可资借鉴的资料。首先，阐述了研究背景及选题的意义，并简要介绍了国内外关于激光在藻类研究领域中的应用概况，指出激光技术在藻类诱变育种领域以及研究的手段和方法中存在的问题，并提出了本论文的研究方案。其次，以Nd：YAP激光、LD激光为光源辐照亚心形扁藻，研究不同激光以及同种激光，不同功率和时间组合辐照对亚心形扁藻的生长速率的影响。结果表明：在本实验中Nd：YAP激光辐照效果优于LD激光，Nd：YAP激光的最佳诱变亚心形扁藻的剂量(10W，55s)。再次，利用紫外可见近红外分光光度计、激光共焦扫描显微镜和透射电子显微镜，研究激光辐照对亚心形扁藻细胞生物量、叶绿体自体荧光强度，叶绿体面积大小和核酸物质的影响。实验结果表明：激光辐照促进扁藻细胞生物量的积累，改善了光合活性，同时也增强了核酸物质的活性。最后，通过测定经激光辐照后亚心形扁藻的生长速率、叶绿素含量、蛋白质浓度、多糖含量和硝酸还原酶活性等生理指标，筛选出两株优质藻株。更多还原

【Abstract】 In this paper, problems in microalgae culturing and solution methods were introduced, then a actual application of laser technology in this field was also studied. Platymonas Subcoydiformis, a most important kind of bait algae was irradiated by Nd:YAP laser and the optimal laser dose, power and duration time were researched. Then the biological effect induced by laser irradiation was studied in virtue of laser scanning confocal microscope, LSCM, UV/VIS/NIR spectrophotometer and transmission electron microscope, TEM simultaneity. At last, individual high quality algae was selected according to the physiological parameters of Platymonas Subcoydiformis. The study provides reference for the further application of laser in algae research.Firstly, the research background and the significance of this dissertation were summarized. Then the status of laser technology application in algae field was introduced. Problems of laser application in algae mutation and breeding were pointed out and then the research method in this study was given.Secondly, Platymonas Subcoydiformis was irradiated by Nd: YAP laser and LD laser. And then the influence on the growth rate of Platymonas Subcoydiformis was studied using different lasers and congeneric laser with different output power and exposure time. The experiment result shows that Nd:YAP laser has a better effect over LD laser with a optimal condition of 10W, 55s.Thirdly, the influence of Nd:YAP laser irradiation on the biology content, auto fluorescence intensity and area of chloroplast as well as the nucleic acid were studied, using UV/VIS/NIR Spectrophotometer, LSCM and TEM. The results have shown that laser irradiation can accelerate the biology quantity accumulation, improve its photosynthesis activity and nucleic acid activity.Finally, after irradiated by Nd:YAP laser, two individual high quality algae were selected from the irradiated group by mensurating their growth rate, the content of chlorophyll, the concentration of protein and the content of amylase as well as the nitratase activity of Platymonas Subcoydiformis.更多还原