【作者】 李英姬；

【导师】 吴光； 崔松彪；

【作者基本信息】 延边大学 ， 内科学， 2007， 硕士

【摘要】 目的：探讨慢性酒中毒患者血清中s-100p蛋白(s-100βprotein s-100β)、髓鞘碱性蛋白(myelin basic protein MBP)浓度的检测结果及其意义。方法：(1)分组1：慢性酒中毒患者52例；对照组正常健康者30例。分组2：将52例慢性酒中毒患者分为酒中毒依赖综合症组32例和酒中毒性幻觉症组20例；对照组为正常健康者30例。分组3：52例慢性酒中毒患者分为酒中毒CT异常组17例；酒中毒CT正常组35例；对照组正常健康者30例。分组4：52例慢性酒中毒患者分为饮酒年限5～10年组16例；饮酒年限10～20年组21例；饮酒年限20～30年组15例；对照组正常健康者30例。(2)用酶联免疫吸附法(eyzyme linkedim munosorbent assay ELISA)检测各组对象血清的S-100β蛋白、MBP浓度，并与30例对照组进行比较。结果：慢性酒中毒患者与对照组血清S-100β蛋白浓度分别为(0.627±0.089)(0.532±0.342)ng／L,有显著差异(p＜0.01)。慢性酒中毒患者酒中毒依赖综合症组和酒中毒性幻觉症组血清S-100β蛋白浓度分别为(0.630±0.082)(0.613±0.089)ng／L，与对照组比较都有显著差异(p＜0.01)。酒中毒性幻觉症组血清MBP浓度(3.421±0.305)ng／L与对照组(2.986±0.392)ng／L比较有显著差异(p＜0.05)。酒中毒CT异常组血清S-100β蛋白、MBP浓度分别为(0.652±0.099)(3.275±0.477)ng／L，与对照组比较有显著差异。酒中毒饮酒10～20年组的血清S-100β蛋白浓度为(0.615±0.041)与对照组比较有显著差异(P＜0.01)，MBP浓度为(3.263±0.286)ng／L，与对照组比较有显著差异(P＜0.05)。酒中毒饮酒20～30年组血清S-100β、MBP浓度分别为(0.729±0.082)(3.578±0.313)ng／L与对照组比较有显著差异(P＜0.01)。结论：慢性酒中毒患者血清S-100β蛋白浓度较对照组增高明显，MBP浓度较对照组有增高趋势，但无统计学差异；血清S-100β蛋白浓度在酒中毒早期开始升高，随饮酒年限增长，临床症状越重变化越明显；慢性酒中毒患者合并脑萎缩时血清S-100β蛋白和MBP浓度增高越明显；血清S-100β蛋白和MBP浓度有可能作为观察慢性酒中毒患者脑损害的生化指标。更多还原

【Abstract】 objective: To investigate the detected result of the chronic alcohollics’ s100B protein in serum and the density of myelin basic protein(MBP) as well as the meaning of doing the investigation.Methods: (1)Group1: 52 chronic alcoholics; compared with 30 normalhealthy persons.Group 2: Divide the 52 chronic alcoholics into two parts: 32dependant alcoholics and 20 alcoholics who have hallucination caused byalcoholism; compared with 30 normal healthy persons.Group 3:52 chronic alcoholics are divided into two parts: 17alcoholics with abnormal CT and 35 alcoholics with normal CT; comparedwith 30 normal healthy persons.Group 4: 52 chronic alcoholics are divided into three partsaccording to the time they have drunk alcohol: 16 of 5-10 years; 21 of10-20 years; 15 of 20-30years; compared with 30 normal healthy persons.(2) Detect s-100B in serum and MBP level of the objects of each groupby using the way of eyzyme-linkedimmunosorbent assay (ELISA), thencompare them with the normal persons’.Result. the density of the chronic alcoholics’ s-100B protein in serumis (627±0.089)while the compared objects’ is (532±0.342)ng/L withsignificance difference (p＜0.01); the density of the dependantalcoholics’ s-100Bprotein is (630±0.082)while the alcoholics who havehallucination has(0.613±0.089)g/Lwith apparent difference (p＜0.01):compared with the versus objects (2.986±0.392).The MBP density of thealcoholics’ who has hallucination is (421±0.305),with Significancedifference (p＜0.05). The density of s-100B and MBP of the alcoholics’with abnormal CT is (0.652±0.099) and (3.275±0.477)ng/L; the densityof s-100B of the alcoholics’ who has drunk for 10-20 years is (0.615±0.041), compared with the versus objects’ (p＜0.01), and the MBPdensity is (3.263±0.286)ng/L with difference(p＜0.05).The density ofs-100B and MBP of the alcoholics’ who have drunk for 20-30 years is (0.729±0.082) and (3.578±0.313)ng/L with significance difference (p＜ 0.01).Conclusion: The density of the s-100B of the chronic alcoholics’ isclearly higher than those of the versus objects’, and the MBP densityhas the tendencyOf increasing though there is no statistic difference;the density of s-100B began to rise in the early years of the alcoholicsdrinking and the numbers increasingly rise with the growing of the ageof the alcoholism: the density of s-100B and MBP apparently rise whenthe chronic alcoholics’ get encephalanalosis; the density of s-100Bprotein and MBP will probably be used as biochemical quota which can helpto observe the destruction in brains of alcoholics’.更多还原