【作者】 罗春丽；

【导师】 张鹏；

【作者基本信息】 北京化工大学 ， 生物化工， 2007， 硕士

【摘要】 辣椒碱类化合物为辣椒中的辛辣成分，主要是由一系列在结构和性质上非常相似的物质构成，其中最具代表性的是辣椒碱。本文以辣椒精为原料，采用现代分离提取技术提取纯化辣椒碱类化合物，主要工作如下：1、对辣椒碱类化合物的色谱检测条件进行优化，确立最佳条件：检测波长为280nm，流动相为甲醇—0.1％磷酸水溶液（V：V=75：25），通过ODS-C18柱（150mm×4.6i．d．,5μm），进样量5μL，柱温40℃，洗脱流速为1.0mL·min^-1，该法适用于目标物含量的精确分析。2、对酸碱—萃取法制备辣椒碱类化合物的工艺进行优化，考察了pH值，碱液质量分数、碱液用量、提取时间、提取温度、料液比及原料用量对目标产物收率的影响，得到最佳工艺条件为：投料比为辣椒精（含辣椒碱1％）：10％NaOH=1g：3ml，在60℃搅拌速度较大情况下提取5h，静置，水相加稀盐酸调pH为5，萃取结晶，收率可达70.64％。3、对乙醇浸提法提取辣椒碱工艺条件进行优化，在单因素的基础上，采用正交实验，得到最佳工艺条件为：以50％的乙醇水溶液作浸提液，料液比为1：3，浸提温度为60℃，浸提时间为3h。4、以含辣椒碱60％的辣椒碱粗品为原料，分别采用离子交换法、酸碱法、萃取结晶法对其进行纯化研究，结论如下：（一）酸碱纯化法所得晶体纯度达94％以上，工艺简单，收率高，较溶剂萃取结晶法为优。（二） 201×7（717）强碱性苯乙烯系阴离子树脂交换法所得晶体纯度可达97.6％，收率为50.7％。更多还原

【Abstract】 Capsaicin compound is the substances responsible for pungency in Capsicum annuum L., which are a series of analogues differing in the structure and character, the most representative is capsaicin. In this paper, on base of the modern technology of extraction and separation, the process of extraction and purification Capsaicin compound from capsicol is studied. The work is described as follows:1. The chromatographic conditions for capsaicin compound were optimized. The optimum detecting condition was determined as follows :MeOH-1%H₃PO₄ solution （V/V=75:25,） system with 1.0mL·min^-1 as the mobile phase, chromatogram column was ODS-C18 （150mm×4.6i.d.,5μm）, injection volume was 5uL,detected at 280nm, 40℃. The method is applicable for accurate analysis on capsaicin compound.2. The optimum process of acid-alkali extracting capsaicin compound was studied. The optimum extraction process was selected with investigating pH, the NaOH solution concentration and dosage, extracting time, temperature, and the ratio of stock to liquid. Optimum extraction condition was determined as follows: Capsicol was dissolved in a solution of 10% NaOH by 1g to 3ml on the inventory rating, extracted about 5 hours in 60℃, stewing , the under stratification pH was medicated to 5 by thin hydrochloric acid ,extraction and crystallization, yield 70.64%.3. The process of extracting capsaicin with ethanol was optimized by orthogonal test. Optimum extraction condition was determined as follows: 50 vol. % ethanol as extracting solvent, stock ratio 1:3 （m/V）, temperature 60℃, time 3 h.4. Ion-exchange method、acid-alkali extraction and crystallization of organic solvent were used to purify capsaicin from crude material containing 60% capsaicin. Results was showed as follows:（一） Capsaicin compound purity was 94% by acid-alkali extraction which was simple and yield was high. It was better than crystallization with organic solvent.（二） Yield of capsaicin compound was 50.7% , purity was 97.6% by 201×7（717） strong basicity styrene anion exchange resin method.更多还原