【作者】 吕海沧；

【导师】 吴德峰； 黄晓蓉；

【作者基本信息】 福建农林大学 ， 生物化学与分子生物学， 2007， 硕士

【摘要】 弧菌是革兰氏阴性、不产孢子的棒状或弧状的细菌，可以运动，许多弧菌在液体培养基条件下生长时具有一个极性鞭毛。弧菌有很多种，其中已经证明对人类具有致病性的弧菌有霍乱弧菌、副溶血弧菌、创伤弧菌三种，其他弧菌都是偶尔会对人类具有致病性，象溶藻弧菌和拟态弧菌等。近年来，越来越多的国家开始关注致病性弧菌的检测，因此有必要建立一种检测方法对致病性弧菌进行检测。在本实验中，我们采用霍乱弧菌的胶原酶基因和霍乱肠毒素基因、拟态弧菌的溶血素基因、副溶血弧菌的不耐热溶血毒素基因、耐热直接溶血毒素基因和相对耐热直接溶血毒素基因、溶藻弧菌的胶原酶基因及创伤弧菌的溶细胞素基因作为扩增序列来检测霍乱弧菌、副溶血弧菌、创伤弧菌、拟态弧菌和溶藻弧菌，建立了一套检测食品中这五种致病性弧菌的多重PCR方法。结果表明，与传统的微生物学方法相比，本方法具有简便快捷的优点，可以用于食品中致病性弧菌的快速检测。更多还原

【Abstract】 Vibrios are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve. They are motile; most have a single polar flagellum when grown in liquid medium.V. cholerae, V. parahaemolyticus and V. vulnificus are well-proved human pathogens. Other Vibrios are occasional human-pathogens, such as V. alginolyticus, V. mimicus and so on. Recently, more and more countries have begun to pay close attention to other Vibrios besides V. cholerae and V. parahaemolyticus. So it is necessary to establish a process to detect the nosogenetic Vibrios.In this study, the V. cholerae collagenase gene and ctxAB gene, V. mimicus heat-labile hemolysin gene, V. parahaemolyticus tlh, tdh and trh genes, V. vulnificus hemolysin gene and V. alginolyticus collagenase gene as genetic markers were used to detect V. cholerae, V. parahaemolyticus, V. mimicus, V. alginolyticus and V. vulnificus. So, a multiplex PCR assay to detect the nosogenetic Vibrios isolated from the food was built up. The results of the method indicate it is more easily, more fastly than the traditional method and can be used to detect the Vibrios in the foods.更多还原