【作者】 韩超；

【导师】 魏成熙；

【作者基本信息】 贵州大学 ， 土壤学， 2007， 硕士

【摘要】 本研究进行了白色马蹄莲切花无土栽培的基质筛选实验，分析了基质中各物理、化学指标对白色马蹄莲切花品质的影响，研究了白色马蹄莲鲜切花在衰败过程中各生理指标的变化规律及自制保鲜剂对这些生理指标变化规律的影响。使用泥炭作为白色马蹄莲切花无土栽培的基质可以取得最佳的切花品质；但若将基质的成本考虑在内，使用下述2种基质可以取得最好的经济效益。基质1：泥炭、蘑菇渣、锯末以1：1：1的体积比混和；基质2：泥炭、蘑菇渣、细沙以1：1：1的体积比混和。在马蹄莲鲜切花的衰败过程中，过氧化物酶（POD）活性、过氧化氢酶（CAT）活性、可溶性蛋白质含量和切花含水率呈现先上升，后下降的变化趋势。丙二醛（MDA）含量、细胞膜透性和活性氧（O₂^-）含量呈现持续升高的变化趋势；超氧化物歧化酶（SOD）活性呈现“M”形变化趋势，经历了2次上升过程和2次下降过程。这些变化规律是研制马蹄莲鲜切花保鲜剂的重要依据。选择含5g/l蔗糖+5g/l聚氧乙烯月桂醚+100mg/l月桂基二甲基氯化氨+10mg/l 6-苄氨基嘌呤（6-BA）+100mg/l Co（NO₃）₂的水溶液作为白色马蹄莲鲜切花的保鲜剂，保鲜期可延长216h，延长了原有观赏期的112.5％。该保鲜剂可显著减慢POD、CAT和SOD活性、可溶性蛋白质含量及切花含水率下降的速率，同时，也使MDA含量、细胞膜透性和超氧自由基含量上升的速率减慢。更多还原

【Abstract】 This study was focus on the selection of culture matrix for calla’s culture.Based on the experiment in vitro, the rule of physical alteration which belongs to the calla in the course of its decline has been studied.The best growth performance and flower qualities were observed for the growth of the two varieties in substance peat, than in matrix T4（the mixture of peat, mushroom debris and sawdust in the volume tatio of 1:1:1） or T6（the mixture of peat, mushroom debris and silver sand in the volume tatio of 1:1:1）.In the course of calla’s decline, the peroxydase（POD）,catalase（CAT）,the content of soluble protein, percentage of moisture of calla’s cut flower rise formerly and decrease latter. the content of propylene glycol（MDA）, cell membrane permeability and the content of superoxide radical（O₂^-） rise consistently. The pattern of the change of superoxide dismutase（SOD）’s activeness is similar with the shape of letter "M". Among the physical alteration mentioned above, some are responsible for the decline of cut flower, others result from the decline of cut flower which are the gist of developing antistaling agent.According to the study, the specilized antistaling agent for calla’s cut flower is the aqueous solution which contains 0.5% cane sugar+ 0.5% polyoxyethylene lauryl ether + 100mg/l lauryl xylidine sal ammoniac+ 10mg/l 6-benzyl aminopurine （6-BA） +100mg/l Co（NO₃）₂ which can prolong the time 216h, the percentage of delay is 112.5%. In general, The activeness of POD,CAT and SOD can be grown, the decline of the content of soluble protein and of the percentage of moisture of calla’s cut flower, the rise of the content of superoxide radical, of MDA and of the cell membrane permeability can changed more slowly with the effection of antistaling agent developed by this study.更多还原