二甲硝咪唑在鸡蛋中的残留研究

【作者】 吴东军；

【导师】 陈眷华；

【作者基本信息】 贵州大学 ， 基础兽医， 2007， 硕士

【摘要】 本实验采用贵州本地小型蛋肉兼用鸡，挑取同一背景，健康且产蛋正常40羽作为实验用鸡。目的：通过检测残留于鸡蛋中的二甲硝咪唑（DMZ）及其主要代谢物羟基二甲硝咪唑（DMZOH）的研究，详细记录在用药期和休药期所有实验用鸡产蛋量和所有鸡蛋个体重量的研究，以期完善DMZ以及咪唑药物的残留研究体系，为临床合理使用DMZ提供参考。方法：本实验通过使用拌料加入DMZ的方法，分别使用不同剂量：150mg．kg^-1、500 mg．kg^-1、1000 mg．kg^-1饲养同一批处于产蛋期的蛋鸡。记录用药前、用药期以及休药期各组产蛋个数；详细称量用药前、用药期以及休药期的所有鸡蛋的重量；使用HPLC法对用药前、用药期以及休药期鸡蛋中的DMZ以及主要代谢产物DMZOH的残留水平的研究。结果：本实验通过数据分析研究表明，拌料加入DMZ的实验组在产蛋数量和鸡蛋重量和对照组无明显差异性，不同剂量的实验组间在产蛋数量和鸡蛋重量两个指标的分析表明也无明显差异性。拌料加入DMZ的实验组药物残留研究表明：在用药期的7日内，所有的实验组中均检测到DMZ和DMZOH，并出现剂量和时间的延长有正相关性，且DMZOH的残留水平均高于DMZ残留水平。在休药期的前7日内，所有实验组的样品检验中，两种药物的残留水平均随时间延长而降低，且有不同剂量组残留水平不同，并且DMZOH残留水平均高于DMZ；但是在休药期的第9日，所有实验组两种药物检测量均降到，〈0.01μg．kg^-1水平。且在第10日均检测不到两种药物的残留。结论：根据本实验研究结果，拌料加入不同剂量DMZ的实验组在用药期和休药期9天内均检测到DMZ和DMZOH。建议蛋鸡在使用DMZ的过程中，蛋和蛋制品应该延长其休药期至停药10天以后方可把其投入市场，严禁把用药期和休药期的蛋以及蛋制品进入流通。更多还原

【Abstract】 40 healthy laying hens were involved in this experiment. Objectives: To get guidance that how to use DMZ reasonably and to perfect the research system of DMZ and imidazole medicine residues through detecting the residue of DMZ and DMZOH in eggs and measuring the numbers and weight of eggs delivered by the hens. Methods: The laying hens were raised with different dosage of forage （150mg/kg、500mg/kg、1000mg/kg） mixed DMZ. Before, during and after using the forage, the weight and quantity of eggs was measured respectively, and the residues of DMZ and its metabolite DMZOH in eggs were detected through the method HP1C separately. Results: There was no difference between the numbers and weights of eggs from the laying hens raised with forage mixed DMZ and that from the control group. Additionally, the difference didn’t exist in the groups raised with different dosage of forage. DMZ and DMZOH could be detected in all experimental groups during using DMZ. The contents were positive related with the dosage of DMZ and time. The remains of DMZ and DMZOH in each group decreased gradually since stopping using DMZ. And the residue of DMZOH was always more than that of DMZ. But till the ninth day after ceasing medicine, the residue of medicine decrease to 0.01ug/kg. Till the 10^th day, DMZ and DMZOH could not be found in each group. Conclusion: According the results of this experiment, DMZ and DMZOH could be found in the experimental groups within 9 days since using medicine and stop using medicine. The egg products and meat products of laying hens raised with DMZ should be sold at least 10 days later when ceasing breeding DMZ.更多还原