【作者】 吕忠海；

【导师】 刘强；

【作者基本信息】 南京农业大学 ， 动物营养与饲料科学， 2006， 硕士

【摘要】 本试验研究了NSP酶对非常规日粮和非常规原料组合日粮养分和NSP消化率的影响，以及NSP酶作用效果在非常规原料组合日粮中的可加性。试验一研究了杂粕型酶制剂对玉米-豆粕日粮、玉米-豆粕-棉粕日粮、玉米-豆粕-菜粕日粮和玉米-豆粕-葵粕日粮，以及稻谷型酶制剂对玉米-豆粕-米糠日粮和小麦型酶制剂对玉米-豆粕-麸皮日粮养分和NSP消化率的影响。试验选择了11周龄健康育成蛋鸡288羽，随机分成12组，每组4个重复，每个重复6羽。结果表明，添加酶制剂后，玉米-豆粕日粮能量代谢率和脂肪消表观化率下降了1.50和2.02个百分点(P＜0.05)，其它养分表观消化率差异不显著，SARA消化率提高了13.7个百分点(P＜0.01)；玉米-豆粕-棉粕日粮能量代谢率和粗纤维消化率分别提高了0.94和9.57个百分点(P＜0.05)，其它养分表观消化率差异不显著，IXYL和IGAL消化率分别提高了4.30和2.13个百分点(P＜0.05)；玉米-豆粕-菜粕日粮的各概略养分表观消化率没有得到明显改善，而INSP消化率提高了2.87个百分点(P＜0.05)；玉米-豆粕-葵粕日粮的粗纤维消化率提高了5.56个百分点(P＜0.05)，TARA和TGLU消化率分别提高了8.28和3.65个百分点(P＜0.05)，其它养分表观消化率改善不明显；玉米-豆粕-米糠日粮的INSP消化率提高了5.68个百分点(P＜0.05)，其它养分表观消化率也未见明显改善；玉米-豆粕-麸皮日粮添加酶制剂后，概略养分和NSP消化率均无明显改善。试验二研究了杂粕型酶制剂对玉米-豆粕基础日粮、玉米-豆粕-棉粕-麸皮日粮、玉米-豆粕-菜粕-米糠日粮和玉米-豆粕-棉粕-菜粕-葵粕日粮养分和NSP消化率的影响，以及杂粕型酶制剂作用效果在这些非常规原料组合日粮中的可加性。试验选择了14周龄健康育成蛋鸡192羽，随机分成8组，每组4个重复，每个重复6羽。结果表明，添加酶制剂后，玉米-豆粕日粮能量代谢率和粗灰分表观消化率分别提高了1.38和3.83个百分点(P＜0.05)，SARA、SGAL和TGAL消化率分别提高了6.38、4.40和6.94个百分点(P＜0.05)：玉米-豆粕-棉粕-麸皮日粮粗纤维消化率提高了8.50个百分点(P＜0.01)，TMF消化率提高了3.65个百分点(P＜0.01)，除干物质和灰分表观消化率外，酶制剂对其它养分表观消化率的效果具有可加性；玉米-豆粕-菜粕-米糠日粮的IARA和IXYL消化率分别提高了10.36和3.64个百分点(P＜0.01)，IMF消化率提高了3.78个百分点(P＜0.05)，其它养分表观消化率均无明显变化，除干物质表观消化率外，酶制剂对该日粮其它养分表观消化率的效果具有可加性；玉米-豆粕-棉粕-菜粕-葵粕日粮SARA和SXYL消化率提高了12.70(P＜0.01)和4.93(P＜0.05)个百分点，其它养分表观消化率未得到明显改善，酶制剂对所有养分表观消化率的影响效果均具有可加性。更多还原

【Abstract】 The purpose of this thesis was to investigate the effects of non-starch polysaccharidesenzyme inclusion on nutrient digestibilities of growing layers in both single and combinednon-conventional diets.In the first experiment, three types of enzyme preparation, i.e., P8306 specialdesigned for un-traditional oil cake/meals, P8302 for paddy by-products, and P8301 forwheat, were used. The digestibilities of gross nutrients and non-starch polysaccharidecomponents after P8306 was added to corn-soybean meal diet, corn-soybeanmeal-cottonseed meal diet, corn-soybean meal-rapeseed meal diet and corn-soybeanmeal-sunflower meal diet, and P8302 to corn-soybean meal-rice bran diet, and P8301 tocorn-soybean meal-wheat bran diet were compared with that of no enzyme contrasts. Twohundred and eighty eight 11-week-age growing layers were randomly divided into 48groupes with 6 layers in each group. Each diet was fed to 4 groups of animals. The resultsshowed that the apparent energy and ether extract (EE) digestibility of corn-soybean mealdiet decreased by 1.50 and 2.02 percentage (P＜0.05), digestibilities of dry matter, crudeprotein and ash also decreased but not significantly, that of SARA increased significantly(P＜0.01) when P8306 was added to the corn-soybean meal diet. The apparent digestibilitiesof energy, crude fibre, IXYL and IGAL increased by 0.95, 9.57, 4.03 and 2.13 percentage(P＜0.05), respectively, that of EE, DM, CP and ash also were improved but without statisticsignificance when P8306 was supplied to the corn-soybean meal-cotton meal diet. TheINSP digestibility was improved by 2.87 percentage (P＜0.05) when P8306 was added to thecorn-soybean meal-rapeseed meal diet. The digestibilities of crude fibre, CP, EE, TARA andTGLU were increased by 5.56, 8.28 and 3.90 percentage (P＜0.05), respectively, whenP8306 was added to the corn-soybean meal-sunflower meal diet. When the corn-soybeanmeal-rice bran diet was supplemented with P8302, the digestibilities of energy, CP, EE andcrude fibre were increased, but that of DM and ash were decreased, although that of INSPincreased by 5.86 percentage (P＜0.05). Both of the CP and NSP digestibility of were notaffected by the inclusion of P8301 into the corn-soybean meal-wheat bran diet.In the second trial, One hundred and ninty two 14-week-age growing layers were randomly divided into 16 groups with 6 layers in each group. The digestibilities of grossnutrients and non-starch polysaccharide components of 4 types of combinedun-conventional diets, i.e., corn-soybean meal diet, wheat bran-cottonseed meal diet, ricebran-rapeseed meal diet and cottonseed meal-rapeseed meal-sunflower meal diet, werecompared with that of P8306 supplemented contrasts. Each diet was fed to 4 groups ofanimals. The results indicated that, after enzyme supplementation, the digestibilities ofenergy, ash, SARA, SGAL and IGAL of corn-soybean meal diet increased by 1.38 (P＜0.05),3.83 (P＜0.05), 6.38 (P＜0.05), 4.40 (P＜0.05), and 6.94 percentage (P＜0.05), respectively.The additive effects of enzyme addition were observed on the improvement of nutrientdigestibility except for DM and ash. The improvement of crude fibre and TMF digestibilityalso was observed in cottonseed meal-wheat bran diet by 8.50 (P＜0.01), and 3.65percentage (P＜0.01). There were additive effects of enzyme addition on the improvement ofnutrient digestibilities except for DM. Enzyme supplementation to corn-soybeanmeal-capseed meal-rice bran diet markedly increased IARA, IXYL, IGAL and IMF by 10.36(P＜0.01), 3.64 (P＜0.01) and 3.78 percentage (P＜0.05). Enzyme supplementation tocorn-soybean meal-cottonseed meal-rapeseed meal-sunflower meal diet increased IARAand IXYL by 12.70 (P＜0.01) and 4.93 percentage (P＜0.05). Some additive effects wereobserved for the improvement of nutrient digestibility with enzymes supplementatiton.更多还原